Toxicogenomic Screening on Dipropyl Phthalate Treated Embryos
Dipropyl phthalate (DPrP), belongs to the phthalate ester (PAEs) family and is widely used as addictive in the plastic products. could be released into the environment through commercial products and food. and it might cause impact to human health by several pathways. Several PAEs had been reporte...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2017
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/32215/1/Toxicogenomic%20Screening%20on%20Dipropyl%20Phthalate%20Treated%20Embryos%2824pgs%29.pdf http://ir.unimas.my/id/eprint/32215/4/Teh%20Kai%20Jia.pdf http://ir.unimas.my/id/eprint/32215/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Dipropyl phthalate (DPrP), belongs to the phthalate ester (PAEs) family and is widely used as addictive in the
plastic products. could be released into the environment through commercial products and food. and it might
cause impact to human health by several pathways. Several PAEs had been reported could act as estrogen and
thyroid endocrine disruptor. The aim of this research is to test the toxic effect of DPrP by using Zebrafish
(Danio rerio) embryo as the animal model. Zebra fish eggs were treated with nine different DPrP concentrations
from 4 hour post-fertilization (hpf) until 96 hpf. DPrP had induced the lethal effect in the embryos. ,vith LC()
was calculated as 5.16 mg/L. Phenotypic analysis using Zebrafish embryo has revealed that DPrP can cause
uninf1ated swimbladder, pericardia! oedema. yolk sac oedema. shorten body length. yolk opaque.
microphthalmia in Zebrafish embryos, and the hatching ability had been affected too. Using toxicogenomic
screening. gene expression of tr that involved in thyroid hormone metabolism were examined by using semiquantitative Reverse-Transcript PCR. Result shown that the band intensity of iur gene was altered and the effect
was dose-dependent. Overall. these results demonstrate that DPrP can cause morphology deformations in
Zebrafish embryo and it might be associated with the effect of thyroid endocrine disruptor. |
|---|