Cover Image

Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL

Piper nigrum L., also known as the pepper plant, produces pepper berries, which is a spice used worldwide. Changes in the natural environment have forced the plant to undergo acclimatization for plant growth. Consequently, un-adapted plants developed unhealthy, stunted growth, affecting the producti...

Full description

Saved in:
Bibliographic Details
Main Authors: Nur Ezzati, Hamdin, Hasnain, Hussain, Chong, Nikson Fatt Ming
Format: Article
Language:English
Published: IOP Publishing 2020
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/32201/1/Identification%20of%20Stress-related%20Proteins%20during%20the%20Growth%20andDevelopment%20of%20Piper%20nigrumL.pdf
http://ir.unimas.my/id/eprint/32201/
https://iopscience.iop.org/article/10.1088/1755-1315/549/1/012072/meta
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.32201
record_format eprints
spelling my.unimas.ir.322012020-10-16T03:14:53Z http://ir.unimas.my/id/eprint/32201/ Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL Nur Ezzati, Hamdin Hasnain, Hussain Chong, Nikson Fatt Ming Q Science (General) QR Microbiology Piper nigrum L., also known as the pepper plant, produces pepper berries, which is a spice used worldwide. Changes in the natural environment have forced the plant to undergo acclimatization for plant growth. Consequently, un-adapted plants developed unhealthy, stunted growth, affecting the production of pepper berries, and thus, causing loss to agriculture. Proteomic analysis using two-dimensional polyacrylamide gel electrophoresis (PAGE) coupled with mass spectrometry was used to compare the differentially expressed proteins between healthy and unhealthy pepper plants. The proteins were successfully extracted from the stems and roots of P. nigrum and the two-dimensional protein profiles were compared. Two-dimensional analysis has indicated significant differences between the healthy and unhealthy parts with 41 and 25 differentially expressed proteins in the stems and roots respectively. A total of 10 differentially expressed proteins, characterized as unique (2) and over-expressed (8) proteins, from both plant parts were selected and sent for identification through matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry. The unique and over-expressed proteins in the unhealthy stems were identified as stress-related small heat shock proteins (sHSPs). These proteins act as a chaperone to protect other proteins against a stressful environment for plant defence and adaptation. Meanwhile, over-expressed proteins from the roots were found to be peroxidase 5, malate dehydrogenase, mitochondrial and gamma-glutamyl phosphate reductase which are involved in oxidative stress, photosynthetic activity, and plant development, respectively. The findings of the differentially expressed proteins in this study, therefore, may create opportunities for further investigations of stress management control and help to improve plantation practices in the future. IOP Publishing 2020 Article PeerReviewed text en http://ir.unimas.my/id/eprint/32201/1/Identification%20of%20Stress-related%20Proteins%20during%20the%20Growth%20andDevelopment%20of%20Piper%20nigrumL.pdf Nur Ezzati, Hamdin and Hasnain, Hussain and Chong, Nikson Fatt Ming (2020) Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL. IOP Conference Series: Earth and Environmental Science, 549. pp. 1-9. https://iopscience.iop.org/article/10.1088/1755-1315/549/1/012072/meta 10.1088/1755-1315/549/1/012072
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Nur Ezzati, Hamdin
Hasnain, Hussain
Chong, Nikson Fatt Ming
Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
description Piper nigrum L., also known as the pepper plant, produces pepper berries, which is a spice used worldwide. Changes in the natural environment have forced the plant to undergo acclimatization for plant growth. Consequently, un-adapted plants developed unhealthy, stunted growth, affecting the production of pepper berries, and thus, causing loss to agriculture. Proteomic analysis using two-dimensional polyacrylamide gel electrophoresis (PAGE) coupled with mass spectrometry was used to compare the differentially expressed proteins between healthy and unhealthy pepper plants. The proteins were successfully extracted from the stems and roots of P. nigrum and the two-dimensional protein profiles were compared. Two-dimensional analysis has indicated significant differences between the healthy and unhealthy parts with 41 and 25 differentially expressed proteins in the stems and roots respectively. A total of 10 differentially expressed proteins, characterized as unique (2) and over-expressed (8) proteins, from both plant parts were selected and sent for identification through matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry. The unique and over-expressed proteins in the unhealthy stems were identified as stress-related small heat shock proteins (sHSPs). These proteins act as a chaperone to protect other proteins against a stressful environment for plant defence and adaptation. Meanwhile, over-expressed proteins from the roots were found to be peroxidase 5, malate dehydrogenase, mitochondrial and gamma-glutamyl phosphate reductase which are involved in oxidative stress, photosynthetic activity, and plant development, respectively. The findings of the differentially expressed proteins in this study, therefore, may create opportunities for further investigations of stress management control and help to improve plantation practices in the future.
format Article
author Nur Ezzati, Hamdin
Hasnain, Hussain
Chong, Nikson Fatt Ming
author_facet Nur Ezzati, Hamdin
Hasnain, Hussain
Chong, Nikson Fatt Ming
author_sort Nur Ezzati, Hamdin
title Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
title_short Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
title_full Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
title_fullStr Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
title_full_unstemmed Identification of Stress-related Proteins during the Growth and Development of Piper nigrumL
title_sort identification of stress-related proteins during the growth and development of piper nigruml
publisher IOP Publishing
publishDate 2020
url http://ir.unimas.my/id/eprint/32201/1/Identification%20of%20Stress-related%20Proteins%20during%20the%20Growth%20andDevelopment%20of%20Piper%20nigrumL.pdf
http://ir.unimas.my/id/eprint/32201/
https://iopscience.iop.org/article/10.1088/1755-1315/549/1/012072/meta
_version_ 1681492678986956800
score 13.211869

Similar Items