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Cloning of green fluorescence protein gene into binary vector

Green Fluorescent Protein (GFP) gene derived from the bioluminescent jellyfish, Aequoera victoria has been widely used in the field of molecular biology where it is utilized as reporter gene of many transformation events. The simplicity of this reporter gene has made it as one of the most stable rep...

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Bibliographic Details
Main Author: Francis, Ruth Grace
Format: Final Year Project Report
Language:English
Published: University Malaysia Sarawak, (UNIMAS) 2013
Subjects:
QR Microbiology
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/3213/7/Ruth%20Grace%20ft.pdf
http://ir.unimas.my/id/eprint/3213/
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spelling my.unimas.ir.32132023-08-28T07:35:46Z http://ir.unimas.my/id/eprint/3213/ Cloning of green fluorescence protein gene into binary vector Francis, Ruth Grace QR Microbiology SB Plant culture Green Fluorescent Protein (GFP) gene derived from the bioluminescent jellyfish, Aequoera victoria has been widely used in the field of molecular biology where it is utilized as reporter gene of many transformation events. The simplicity of this reporter gene has made it as one of the most stable reporter gene in plant transformation. Through times, a lot of researches have been done to study the applications of gfp gene in plants. In this project, a modification has been done on the genetic material of the binary vector, pGSA1131 which involved the removal of the destructive gus gene via restriction enzyme digestion followed by the blunting of gfp gene and vector ends, ligation, transformation of ligated vector and finally analysis of transformants. Based on the analysis, it is said that the cloning of the gfp gene obtained from plasmid MCB30 into binary vector pGSA1131 was not successful. University Malaysia Sarawak, (UNIMAS) 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/3213/7/Ruth%20Grace%20ft.pdf Francis, Ruth Grace (2013) Cloning of green fluorescence protein gene into binary vector. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
SB Plant culture
spellingShingle QR Microbiology
SB Plant culture
Francis, Ruth Grace
Cloning of green fluorescence protein gene into binary vector
description Green Fluorescent Protein (GFP) gene derived from the bioluminescent jellyfish, Aequoera victoria has been widely used in the field of molecular biology where it is utilized as reporter gene of many transformation events. The simplicity of this reporter gene has made it as one of the most stable reporter gene in plant transformation. Through times, a lot of researches have been done to study the applications of gfp gene in plants. In this project, a modification has been done on the genetic material of the binary vector, pGSA1131 which involved the removal of the destructive gus gene via restriction enzyme digestion followed by the blunting of gfp gene and vector ends, ligation, transformation of ligated vector and finally analysis of transformants. Based on the analysis, it is said that the cloning of the gfp gene obtained from plasmid MCB30 into binary vector pGSA1131 was not successful.
format Final Year Project Report
author Francis, Ruth Grace
author_facet Francis, Ruth Grace
author_sort Francis, Ruth Grace
title Cloning of green fluorescence protein gene into binary vector
title_short Cloning of green fluorescence protein gene into binary vector
title_full Cloning of green fluorescence protein gene into binary vector
title_fullStr Cloning of green fluorescence protein gene into binary vector
title_full_unstemmed Cloning of green fluorescence protein gene into binary vector
title_sort cloning of green fluorescence protein gene into binary vector
publisher University Malaysia Sarawak, (UNIMAS)
publishDate 2013
url http://ir.unimas.my/id/eprint/3213/7/Ruth%20Grace%20ft.pdf
http://ir.unimas.my/id/eprint/3213/
_version_ 1775627152661676032
score 13.211869

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