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Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)

Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical forest tree. It has high commercial value for timber production and possesses several medical values, such as anthelmintic and astringent production. The intention of this study was to isolate and characterize the parti...

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Main Author: Ng, Siew San
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, UNIMAS 2013
Subjects:
R Medicine (General)
RZ Other systems of medicine
Online Access:http://ir.unimas.my/id/eprint/3185/1/Ng%20Siew%20San%2024pgs.pdf
http://ir.unimas.my/id/eprint/3185/3/Ng%20Siew%20San%20ft.pdf
http://ir.unimas.my/id/eprint/3185/
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spelling my.unimas.ir.31852023-10-19T09:19:01Z http://ir.unimas.my/id/eprint/3185/ Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba) Ng, Siew San R Medicine (General) RZ Other systems of medicine Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical forest tree. It has high commercial value for timber production and possesses several medical values, such as anthelmintic and astringent production. The intention of this study was to isolate and characterize the partial complementary DNA (cDNA) of Dehydroascorbate reductase (DHAR) gene by using RT-PCR in Kelampayan. Dehydroascorbate reductase (DHAR) is an important reducing enzyme in ascorbate-glutathione cycle. It maintains the level of ascorbate in the plants. Ascorbate (AsA) protects the plants from environmental stress.It also involved in controlling the appropriate signaling in guard cell. In this study, ribonucleic acid (RNA) of N. cadamba was extracted from fresh young leaves of Kelampayan and the purified polymerase chain reaction (PCR) product was sent for direct sequencing. Results showed that the partial cDNA of N.cadamba was successfully isolated. The sequence had high similarity when compared with DHAR in other species using BLASTn and BLASTp. The partial cDNA of DHAR gene isolated was the middle region when compared to the complete cDNA of DHAR in Populus tementosa. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/3185/1/Ng%20Siew%20San%2024pgs.pdf text en http://ir.unimas.my/id/eprint/3185/3/Ng%20Siew%20San%20ft.pdf Ng, Siew San (2013) Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba). [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic R Medicine (General)
RZ Other systems of medicine
spellingShingle R Medicine (General)
RZ Other systems of medicine
Ng, Siew San
Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
description Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical forest tree. It has high commercial value for timber production and possesses several medical values, such as anthelmintic and astringent production. The intention of this study was to isolate and characterize the partial complementary DNA (cDNA) of Dehydroascorbate reductase (DHAR) gene by using RT-PCR in Kelampayan. Dehydroascorbate reductase (DHAR) is an important reducing enzyme in ascorbate-glutathione cycle. It maintains the level of ascorbate in the plants. Ascorbate (AsA) protects the plants from environmental stress.It also involved in controlling the appropriate signaling in guard cell. In this study, ribonucleic acid (RNA) of N. cadamba was extracted from fresh young leaves of Kelampayan and the purified polymerase chain reaction (PCR) product was sent for direct sequencing. Results showed that the partial cDNA of N.cadamba was successfully isolated. The sequence had high similarity when compared with DHAR in other species using BLASTn and BLASTp. The partial cDNA of DHAR gene isolated was the middle region when compared to the complete cDNA of DHAR in Populus tementosa.
format Final Year Project Report
author Ng, Siew San
author_facet Ng, Siew San
author_sort Ng, Siew San
title Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
title_short Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
title_full Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
title_fullStr Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
title_full_unstemmed Molecular cloning and characterization of partial cDNA encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
title_sort molecular cloning and characterization of partial cdna encoding for dehydroascorbate reductase from kelampayan (neolamarckia cadamba)
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2013
url http://ir.unimas.my/id/eprint/3185/1/Ng%20Siew%20San%2024pgs.pdf
http://ir.unimas.my/id/eprint/3185/3/Ng%20Siew%20San%20ft.pdf
http://ir.unimas.my/id/eprint/3185/
_version_ 1781710274656468992
score 13.211869

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