Staff View: The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses

The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses

Glucosinolates are defense-related secondary metabolites found in Brassicaceae. When Brassicaceae come under attack, glucosinolates are hydrolyzed into different forms of glucosinolate hydrolysis products (GHPs). Among the GHPs, isothiocyanates are the most comprehensively characterized defensive co...

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Main Authors:	Yeo, Freddy Kuok San, Ting, Hieng Ming, Cheah, Boon, Chen, Yu Cheng, Yeh, Pei-Min, Cheng, Chiu Ping, Vie, Ane Kjersti, Rohloff, Jen, Winge, Per, Bones, Atle M., Kissen, Ralph
Format:	Article
Language:	English
Published:	Frontiers Media 2020
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/31432/1/role.pdf http://ir.unimas.my/id/eprint/31432/ https://www.frontiersin.org/articles/10.3389/fpls.2020.00257/full#:~:text=This%20research%20indicates%20that%20the,in%20guard%20cells%20of%20A.
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id	my.unimas.ir.31432
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spelling	my.unimas.ir.314322021-06-05T06:04:20Z http://ir.unimas.my/id/eprint/31432/ The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses Yeo, Freddy Kuok San Ting, Hieng Ming Cheah, Boon Chen, Yu Cheng Yeh, Pei-Min Cheng, Chiu Ping Vie, Ane Kjersti Rohloff, Jen Winge, Per Bones, Atle M. Kissen, Ralph SB Plant culture Glucosinolates are defense-related secondary metabolites found in Brassicaceae. When Brassicaceae come under attack, glucosinolates are hydrolyzed into different forms of glucosinolate hydrolysis products (GHPs). Among the GHPs, isothiocyanates are the most comprehensively characterized defensive compounds, whereas the functional study of nitriles, another group of GHP, is still limited. Therefore, this study investigates whether 3-butenenitrile (3BN), a nitrile, can trigger the signaling pathways involved in the regulation of defense responses in Arabidopsis thaliana against biotic stresses. Briefly, the methodology is divided into three stages, (i) evaluate the physiological and biochemical effects of exogenous 3BN treatment on Arabidopsis, (ii) determine the metabolites involved in 3BN-mediated defense responses in Arabidopsis, and (iii) assess whether a 3BN treatment can enhance the disease tolerance of Arabidopsis against necrotrophic pathogens. As a result, a 2.5 mM 3BN treatment caused lesion formation in Arabidopsis Columbia (Col-0) plants, a process found to be modulated by nitric oxide (NO). Metabolite profiling revealed an increased production of soluble sugars, Krebs cycle associated carboxylic acids and amino acids in Arabidopsis upon a 2.5 mM 3BN treatment, presumably via NO action. Primary metabolites such as sugars and amino acids are known to be crucial components in modulating plant defense responses Frontiers Media 2020 Article PeerReviewed text en http://ir.unimas.my/id/eprint/31432/1/role.pdf Yeo, Freddy Kuok San and Ting, Hieng Ming and Cheah, Boon and Chen, Yu Cheng and Yeh, Pei-Min and Cheng, Chiu Ping and Vie, Ane Kjersti and Rohloff, Jen and Winge, Per and Bones, Atle M. and Kissen, Ralph (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science, 11 (257). pp. 1-18. ISSN 1664-462X https://www.frontiersin.org/articles/10.3389/fpls.2020.00257/full#:~:text=This%20research%20indicates%20that%20the,in%20guard%20cells%20of%20A. DOI: 10.3389/fpls.2020.00257
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Yeo, Freddy Kuok San Ting, Hieng Ming Cheah, Boon Chen, Yu Cheng Yeh, Pei-Min Cheng, Chiu Ping Vie, Ane Kjersti Rohloff, Jen Winge, Per Bones, Atle M. Kissen, Ralph The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
description	Glucosinolates are defense-related secondary metabolites found in Brassicaceae. When Brassicaceae come under attack, glucosinolates are hydrolyzed into different forms of glucosinolate hydrolysis products (GHPs). Among the GHPs, isothiocyanates are the most comprehensively characterized defensive compounds, whereas the functional study of nitriles, another group of GHP, is still limited. Therefore, this study investigates whether 3-butenenitrile (3BN), a nitrile, can trigger the signaling pathways involved in the regulation of defense responses in Arabidopsis thaliana against biotic stresses. Briefly, the methodology is divided into three stages, (i) evaluate the physiological and biochemical effects of exogenous 3BN treatment on Arabidopsis, (ii) determine the metabolites involved in 3BN-mediated defense responses in Arabidopsis, and (iii) assess whether a 3BN treatment can enhance the disease tolerance of Arabidopsis against necrotrophic pathogens. As a result, a 2.5 mM 3BN treatment caused lesion formation in Arabidopsis Columbia (Col-0) plants, a process found to be modulated by nitric oxide (NO). Metabolite profiling revealed an increased production of soluble sugars, Krebs cycle associated carboxylic acids and amino acids in Arabidopsis upon a 2.5 mM 3BN treatment, presumably via NO action. Primary metabolites such as sugars and amino acids are known to be crucial components in modulating plant defense responses
format	Article
author	Yeo, Freddy Kuok San Ting, Hieng Ming Cheah, Boon Chen, Yu Cheng Yeh, Pei-Min Cheng, Chiu Ping Vie, Ane Kjersti Rohloff, Jen Winge, Per Bones, Atle M. Kissen, Ralph
author_facet	Yeo, Freddy Kuok San Ting, Hieng Ming Cheah, Boon Chen, Yu Cheng Yeh, Pei-Min Cheng, Chiu Ping Vie, Ane Kjersti Rohloff, Jen Winge, Per Bones, Atle M. Kissen, Ralph
author_sort	Yeo, Freddy Kuok San
title	The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
title_short	The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
title_full	The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
title_fullStr	The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
title_full_unstemmed	The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses
title_sort	role of a glucosinolate-derived nitrile in plant immune responses
publisher	Frontiers Media
publishDate	2020
url	http://ir.unimas.my/id/eprint/31432/1/role.pdf http://ir.unimas.my/id/eprint/31432/ https://www.frontiersin.org/articles/10.3389/fpls.2020.00257/full#:~:text=This%20research%20indicates%20that%20the,in%20guard%20cells%20of%20A.
_version_	1702173266413617152
score	13.211869

The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses

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