Cover Image

Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants

Aquilaria microcarpa BailI. is a tree species from family Thymelaeaceae. It is one of the tree species producing agarwood which is highly demanded commercially. Agarwood is a fragrant wood utilized as incense, perfume and medicinal product. Illegal poaching and over-exploitation have led to rapid...

Full description

Saved in:
Bibliographic Details
Main Author: Ng, Sook Chiun
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/31368/1/Ng%20Sook%20ft.pdf
http://ir.unimas.my/id/eprint/31368/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.31368
record_format eprints
spelling my.unimas.ir.313682024-06-13T09:16:58Z http://ir.unimas.my/id/eprint/31368/ Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants Ng, Sook Chiun SB Plant culture Aquilaria microcarpa BailI. is a tree species from family Thymelaeaceae. It is one of the tree species producing agarwood which is highly demanded commercially. Agarwood is a fragrant wood utilized as incense, perfume and medicinal product. Illegal poaching and over-exploitation have led to rapid declination of this species. Thus, mass propagation of A. microcarpa is needed for large scale planting. The objective of present study was attempted to establish a micropropagation protocol for A. microcarpa. Shoots tips from two years old seedlings were left in running water for one hour, dipped in 70% ethanol for 30 seconds, surface sterilized with 15% Clorox® added with 'Tween 20' for 15 minutes with gentle agitation and subsequently soaked in the various fungicides for different exposure times. The explants were cultured in WPM supplemented with or without biocide and antibiotic of several concentrations. The axenic explants were then cultured on WPM supplemented with BAP alone or in combination with IBA. Most of the shoot tip explants sprouted shoots or shoot-like-structures within two months of culture. The explants started to elongate within two weeks after initiation of cultured. Medium supplemented with 3.0 mglL BAP was most effective to induce shoot proliferation for this species. However, medium supplemented with 3.0 mglL BAP and 1.0 mgfL IBA was failed to induce shoot multiplication although it elongated the shoots. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/31368/1/Ng%20Sook%20ft.pdf Ng, Sook Chiun (2011) Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Ng, Sook Chiun
Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
description Aquilaria microcarpa BailI. is a tree species from family Thymelaeaceae. It is one of the tree species producing agarwood which is highly demanded commercially. Agarwood is a fragrant wood utilized as incense, perfume and medicinal product. Illegal poaching and over-exploitation have led to rapid declination of this species. Thus, mass propagation of A. microcarpa is needed for large scale planting. The objective of present study was attempted to establish a micropropagation protocol for A. microcarpa. Shoots tips from two years old seedlings were left in running water for one hour, dipped in 70% ethanol for 30 seconds, surface sterilized with 15% Clorox® added with 'Tween 20' for 15 minutes with gentle agitation and subsequently soaked in the various fungicides for different exposure times. The explants were cultured in WPM supplemented with or without biocide and antibiotic of several concentrations. The axenic explants were then cultured on WPM supplemented with BAP alone or in combination with IBA. Most of the shoot tip explants sprouted shoots or shoot-like-structures within two months of culture. The explants started to elongate within two weeks after initiation of cultured. Medium supplemented with 3.0 mglL BAP was most effective to induce shoot proliferation for this species. However, medium supplemented with 3.0 mglL BAP and 1.0 mgfL IBA was failed to induce shoot multiplication although it elongated the shoots.
format Final Year Project Report
author Ng, Sook Chiun
author_facet Ng, Sook Chiun
author_sort Ng, Sook Chiun
title Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
title_short Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
title_full Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
title_fullStr Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
title_full_unstemmed Induction of Direct Organogenesis in Aquilaria microcarpa Baill. Using Shoot Explants
title_sort induction of direct organogenesis in aquilaria microcarpa baill. using shoot explants
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/31368/1/Ng%20Sook%20ft.pdf
http://ir.unimas.my/id/eprint/31368/
_version_ 1802981805907771392
score 13.211869

Similar Items