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Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense

The white-rot fungus, Ganodenna boninense has been one of important pathogens in causing the basal stem rot (BSR) disease in oil palm and other perennial tree; especially in Malaysia. This study was conducted to use beneficial microorganisms as the antagonists in the dual culture tests against the p...

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Main Author: Norsyafiqah, Jaafar
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2014
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/30576/2/Norsyafiqah%20Jaafar%28Fulltext%29.pdf
http://ir.unimas.my/id/eprint/30576/
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spelling my.unimas.ir.305762023-10-05T07:58:11Z http://ir.unimas.my/id/eprint/30576/ Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense Norsyafiqah, Jaafar Q Science (General) The white-rot fungus, Ganodenna boninense has been one of important pathogens in causing the basal stem rot (BSR) disease in oil palm and other perennial tree; especially in Malaysia. This study was conducted to use beneficial microorganisms as the antagonists in the dual culture tests against the pathogen, G. boninense. Initially, each of the microorganism isolate and the pathogen were inoculated simultaneously on media in the same Petri dish, opposite to each other with initial distance of 5 em to determine their interaction effect. The ability of the antagonists to inhibit the growth ofthe pathogen was assessed by measuring the radius of the G. boninense colony towards the antagonist after seven days of culture. A total of30 fungal isolates reduced the growth of the pathogen but neither of the two bacteria isolates used in this study showed effect on G. boninense growth. The most effective fungal isolate to inhibit growth of the pathogen was isolate 1864 with PIRG 97%. Formulation using eight selected isolates from the dual culture tests with PIRG >90% were tested on the pathogen. Treatment inoculated simultaneously with the pathogen (T2) was more effective against G. boninense compared to the treatment applied after five days growth of the pathogen (Tl) as from day 3 of culture, the pathogen had stopped growing. Further research on using beneficial microorganisms against the pathogen should be continued for its big potential as biological control agents. Universiti Malaysia Sarawak (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/30576/2/Norsyafiqah%20Jaafar%28Fulltext%29.pdf Norsyafiqah, Jaafar (2014) Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Norsyafiqah, Jaafar
Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
description The white-rot fungus, Ganodenna boninense has been one of important pathogens in causing the basal stem rot (BSR) disease in oil palm and other perennial tree; especially in Malaysia. This study was conducted to use beneficial microorganisms as the antagonists in the dual culture tests against the pathogen, G. boninense. Initially, each of the microorganism isolate and the pathogen were inoculated simultaneously on media in the same Petri dish, opposite to each other with initial distance of 5 em to determine their interaction effect. The ability of the antagonists to inhibit the growth ofthe pathogen was assessed by measuring the radius of the G. boninense colony towards the antagonist after seven days of culture. A total of30 fungal isolates reduced the growth of the pathogen but neither of the two bacteria isolates used in this study showed effect on G. boninense growth. The most effective fungal isolate to inhibit growth of the pathogen was isolate 1864 with PIRG 97%. Formulation using eight selected isolates from the dual culture tests with PIRG >90% were tested on the pathogen. Treatment inoculated simultaneously with the pathogen (T2) was more effective against G. boninense compared to the treatment applied after five days growth of the pathogen (Tl) as from day 3 of culture, the pathogen had stopped growing. Further research on using beneficial microorganisms against the pathogen should be continued for its big potential as biological control agents.
format Final Year Project Report
author Norsyafiqah, Jaafar
author_facet Norsyafiqah, Jaafar
author_sort Norsyafiqah, Jaafar
title Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
title_short Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
title_full Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
title_fullStr Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
title_full_unstemmed Effectiveness of Formulation using Different Microorganisms as Biocides against Ganoderma boninense
title_sort effectiveness of formulation using different microorganisms as biocides against ganoderma boninense
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/30576/2/Norsyafiqah%20Jaafar%28Fulltext%29.pdf
http://ir.unimas.my/id/eprint/30576/
_version_ 1779150667474010112
score 13.211869

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