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Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers

Jatropha curcas L. (Euphorbiaceae), the oil bearing species is native to Central America having significant economic importance due to its promising potential in biodiesel production. Analysis of genetic diversity of Jatropha curcas L. has become vital due to lack of molecular work done for this spe...

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Main Author: Nurhaliza, Mohamad Shahidin
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2010
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/30505/2/Genetic%20diversity%20among%20Jatropha%20Curcas%20L.%20detected%20by%20random%20amplified%20polymorphic%20DNA%20%28RAPD%29%20markers%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/30505/
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spelling my.unimas.ir.305052024-01-08T08:13:45Z http://ir.unimas.my/id/eprint/30505/ Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers Nurhaliza, Mohamad Shahidin SB Plant culture Jatropha curcas L. (Euphorbiaceae), the oil bearing species is native to Central America having significant economic importance due to its promising potential in biodiesel production. Analysis of genetic diversity of Jatropha curcas L. has become vital due to lack of molecular work done for this species and also due to limited information available for genetic improvement of this crop. Hence, this study was undertaken with an aim to access the extent of genetic variability and genetic relationship among different Jatropha curcas L. accessions from Peninsular Malaysia, Sarawak and Kalimantan. Seven accessions of Jatropha curcas L. were analyzed using Random Amplified Polymorphic DNA (RAPD) marker techniques based on polymerase chain reaction (PCR). Genomic DNA from seven accessions of Jatropha curcas L. was successfully extracted. From the results obtained, the optimum annealing temperature was 33.4°C while the optimum DNA concentration was 20 ng. The optimum annealing temperature and optimum DNA concentration was obtained after several attempts. Optimization of MgCl2 concentration was failed due to failure in amplification. PCR amplification was failed because of failure in optimization of PCR parameters. Genetic variability and genetic relationships between seven accessions of Jatropha curcas L. were not obtained. However, this study could provide preliminary molecular data for the next attempt of genetic diversity study of Jatropha curcas L. Universiti Malaysia Sarawak (UNIMAS) 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/30505/2/Genetic%20diversity%20among%20Jatropha%20Curcas%20L.%20detected%20by%20random%20amplified%20polymorphic%20DNA%20%28RAPD%29%20markers%20%28fulltext%29.pdf Nurhaliza, Mohamad Shahidin (2010) Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Nurhaliza, Mohamad Shahidin
Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
description Jatropha curcas L. (Euphorbiaceae), the oil bearing species is native to Central America having significant economic importance due to its promising potential in biodiesel production. Analysis of genetic diversity of Jatropha curcas L. has become vital due to lack of molecular work done for this species and also due to limited information available for genetic improvement of this crop. Hence, this study was undertaken with an aim to access the extent of genetic variability and genetic relationship among different Jatropha curcas L. accessions from Peninsular Malaysia, Sarawak and Kalimantan. Seven accessions of Jatropha curcas L. were analyzed using Random Amplified Polymorphic DNA (RAPD) marker techniques based on polymerase chain reaction (PCR). Genomic DNA from seven accessions of Jatropha curcas L. was successfully extracted. From the results obtained, the optimum annealing temperature was 33.4°C while the optimum DNA concentration was 20 ng. The optimum annealing temperature and optimum DNA concentration was obtained after several attempts. Optimization of MgCl2 concentration was failed due to failure in amplification. PCR amplification was failed because of failure in optimization of PCR parameters. Genetic variability and genetic relationships between seven accessions of Jatropha curcas L. were not obtained. However, this study could provide preliminary molecular data for the next attempt of genetic diversity study of Jatropha curcas L.
format Final Year Project Report
author Nurhaliza, Mohamad Shahidin
author_facet Nurhaliza, Mohamad Shahidin
author_sort Nurhaliza, Mohamad Shahidin
title Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
title_short Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
title_full Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
title_fullStr Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
title_full_unstemmed Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers
title_sort genetic diversity among jatropha curcas l. detected by random amplified polymorphic dna (rapd) markers
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2010
url http://ir.unimas.my/id/eprint/30505/2/Genetic%20diversity%20among%20Jatropha%20Curcas%20L.%20detected%20by%20random%20amplified%20polymorphic%20DNA%20%28RAPD%29%20markers%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/30505/
_version_ 1787519567362260992
score 13.211869

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