Staff View: The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.

The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.

Objective: To investigate the allele and genotype frequencies of NFKBI -94 ins/de! ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of...

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Main Authors:	Mohd Suzairi, M.S., Tan, S.C., Ahmad Aizat, Abdul Aziz, Mohd Aminudin, Mustapha, Siti Nurfatimah, Mohd Shahpudin, Andee, Z.D., Ankathil, Ravindran
Format:	Article
Language:	English
Published:	Elsevier Science, Ltd. 2013
Subjects:	Q Science (General) R Medicine (General)
Online Access:	http://ir.unimas.my/id/eprint/30234/1/The%20functional%20.pdf http://ir.unimas.my/id/eprint/30234/ https://www.sciencedirect.com/science/article/abs/pii/S1877782113000945 https://doi.org/10.1016/j.canep.2013.05.007
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spelling	my.unimas.ir.302342023-07-31T03:37:42Z http://ir.unimas.my/id/eprint/30234/ The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer. Mohd Suzairi, M.S. Tan, S.C. Ahmad Aizat, Abdul Aziz Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Andee, Z.D. Ankathil, Ravindran Q Science (General) R Medicine (General) Objective: To investigate the allele and genotype frequencies of NFKBI -94 ins/de! ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKBI -94 ins/de! ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (Cls) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous ( del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01 ). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR= 2.42, 95% CI= 1.24-4.73, P < 0.01 ). Conclusions: The variant allele of NFKBI -94 ins/de! ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population. Elsevier Science, Ltd. 2013-06 Article PeerReviewed text en http://ir.unimas.my/id/eprint/30234/1/The%20functional%20.pdf Mohd Suzairi, M.S. and Tan, S.C. and Ahmad Aizat, Abdul Aziz and Mohd Aminudin, Mustapha and Siti Nurfatimah, Mohd Shahpudin and Andee, Z.D. and Ankathil, Ravindran (2013) The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer. Cancer Epidemiology, 37 (5). pp. 634-638. ISSN 1877-7821 https://www.sciencedirect.com/science/article/abs/pii/S1877782113000945 https://doi.org/10.1016/j.canep.2013.05.007
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) R Medicine (General)
spellingShingle	Q Science (General) R Medicine (General) Mohd Suzairi, M.S. Tan, S.C. Ahmad Aizat, Abdul Aziz Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Andee, Z.D. Ankathil, Ravindran The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
description	Objective: To investigate the allele and genotype frequencies of NFKBI -94 ins/de! ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKBI -94 ins/de! ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (Cls) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous ( del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01 ). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR= 2.42, 95% CI= 1.24-4.73, P < 0.01 ). Conclusions: The variant allele of NFKBI -94 ins/de! ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population.
format	Article
author	Mohd Suzairi, M.S. Tan, S.C. Ahmad Aizat, Abdul Aziz Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Andee, Z.D. Ankathil, Ravindran
author_facet	Mohd Suzairi, M.S. Tan, S.C. Ahmad Aizat, Abdul Aziz Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Andee, Z.D. Ankathil, Ravindran
author_sort	Mohd Suzairi, M.S.
title	The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
title_short	The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
title_full	The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
title_fullStr	The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
title_full_unstemmed	The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.
title_sort	functional -94 insertion/deletion attg polymorphism in the promoter region of nfkb1 gene increases the risk of sporadic colorectal cancer.
publisher	Elsevier Science, Ltd.
publishDate	2013
url	http://ir.unimas.my/id/eprint/30234/1/The%20functional%20.pdf http://ir.unimas.my/id/eprint/30234/ https://www.sciencedirect.com/science/article/abs/pii/S1877782113000945 https://doi.org/10.1016/j.canep.2013.05.007
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score	13.211869

The functional -94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer.

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