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TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination

The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the...

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Main Authors: Bhawani, Showkat Ahmad, Sufia, Hena, Ali, Mohammad
Format: E-Article
Language:English
Published: NISCAIR-CSIR, India 2006
Subjects:
QD Chemistry
TP Chemical technology
Online Access:http://ir.unimas.my/id/eprint/29697/1/TLC%20separation%20of%20L-tryptophan%20using%20microemulsion%20mobile%20phase%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/29697/
http://nopr.niscair.res.in/handle/123456789/20019
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spelling my.unimas.ir.296972020-05-13T04:14:02Z http://ir.unimas.my/id/eprint/29697/ TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination Bhawani, Showkat Ahmad Sufia, Hena Ali, Mohammad QD Chemistry TP Chemical technology The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the presence of metal cation impurities. The proposed method is applicable to the identification and separation of L-tryptophan from drug samples such as Astymin -m (Forte), Astymin (liquid) and Alamin (Forte). The separation up to 10μg of L-tryptophan from milligram quantities (up to 0.77 mg) of other amino acids has also been realized. The quantitative estimation of L-tryptophan by spectrophotometry (λmax 570 nm) after separation from other amino acids has been done. The recovery of tryptophan is around 98% and its limit of detection on silica gel layer is 0.13 μg. NISCAIR-CSIR, India 2006 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/29697/1/TLC%20separation%20of%20L-tryptophan%20using%20microemulsion%20mobile%20phase%20-%20Copy.pdf Bhawani, Showkat Ahmad and Sufia, Hena and Ali, Mohammad (2006) TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination. Indian Journal of Chemistry -Section A (IJC-A), 45A. pp. 1663-1666. ISSN 0975-0975 http://nopr.niscair.res.in/handle/123456789/20019
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
TP Chemical technology
spellingShingle QD Chemistry
TP Chemical technology
Bhawani, Showkat Ahmad
Sufia, Hena
Ali, Mohammad
TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
description The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the presence of metal cation impurities. The proposed method is applicable to the identification and separation of L-tryptophan from drug samples such as Astymin -m (Forte), Astymin (liquid) and Alamin (Forte). The separation up to 10μg of L-tryptophan from milligram quantities (up to 0.77 mg) of other amino acids has also been realized. The quantitative estimation of L-tryptophan by spectrophotometry (λmax 570 nm) after separation from other amino acids has been done. The recovery of tryptophan is around 98% and its limit of detection on silica gel layer is 0.13 μg.
format E-Article
author Bhawani, Showkat Ahmad
Sufia, Hena
Ali, Mohammad
author_facet Bhawani, Showkat Ahmad
Sufia, Hena
Ali, Mohammad
author_sort Bhawani, Showkat Ahmad
title TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
title_short TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
title_full TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
title_fullStr TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
title_full_unstemmed TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
title_sort tlc separation of l-tryptophan using microemulsion mobile phase and its spectrophotometric determination
publisher NISCAIR-CSIR, India
publishDate 2006
url http://ir.unimas.my/id/eprint/29697/1/TLC%20separation%20of%20L-tryptophan%20using%20microemulsion%20mobile%20phase%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/29697/
http://nopr.niscair.res.in/handle/123456789/20019
_version_ 1669010449566269440
score 13.211869

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