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Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)

This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed bas...

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Main Author: Lau, Jennifer Siew Kee
Format: Thesis
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2001
Subjects:
QK Botany
Online Access:http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf
http://ir.unimas.my/id/eprint/27918/
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spelling my.unimas.ir.279182023-03-09T07:46:38Z http://ir.unimas.my/id/eprint/27918/ Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu) Lau, Jennifer Siew Kee QK Botany This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed based on the conserved regions of the GBSS. The RT-PCR product obtained, 917 bp in size, was cloned and sequenced. Homology search by using the BLASTX search program confirmed that the RT-PCR product consists of part of the GBSS gene, with the deduced amino acid sequence sharing 68-7:Z01o identities with GBSS of rice, potato, maize, wheat and cassava. A pair of specific internal primers was then designed based on the RT-PCR product. These primers were used to amplify the corresponding genomic DNA region via a genomic PCR method. A 2,008 bp genomic PCR product was generated by this primer set. Cloning and sequencing of the genomic PCR product confinned that it consists of a partial genomic GBSS gene. This genomic PCR product was then used as a probe in an attempt to isolate the entire genomic locus ofthe sago GBSS via Southern hybridization. Universiti Malaysia Sarawak (UNIMAS) 2001 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf Lau, Jennifer Siew Kee (2001) Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu). Masters thesis, Universiti Malaysia Sarawak (UNIMAS).
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QK Botany
spellingShingle QK Botany
Lau, Jennifer Siew Kee
Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
description This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed based on the conserved regions of the GBSS. The RT-PCR product obtained, 917 bp in size, was cloned and sequenced. Homology search by using the BLASTX search program confirmed that the RT-PCR product consists of part of the GBSS gene, with the deduced amino acid sequence sharing 68-7:Z01o identities with GBSS of rice, potato, maize, wheat and cassava. A pair of specific internal primers was then designed based on the RT-PCR product. These primers were used to amplify the corresponding genomic DNA region via a genomic PCR method. A 2,008 bp genomic PCR product was generated by this primer set. Cloning and sequencing of the genomic PCR product confinned that it consists of a partial genomic GBSS gene. This genomic PCR product was then used as a probe in an attempt to isolate the entire genomic locus ofthe sago GBSS via Southern hybridization.
format Thesis
author Lau, Jennifer Siew Kee
author_facet Lau, Jennifer Siew Kee
author_sort Lau, Jennifer Siew Kee
title Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_short Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_full Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_fullStr Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_full_unstemmed Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_sort isolation and characterization of near complete cdna and genomic sequences coding for granule-bound starch synthase in sago palm (metroxylon sagu)
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2001
url http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf
http://ir.unimas.my/id/eprint/27918/
_version_ 1761623531098996736
score 13.211869

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