Cover Image

Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions

Neolamarckia cadamba belongs to Rubiaceae family which is a fast-growing timber species. It is widely used as traditional medicines and suitable for reforestation. Demand for Neolamarckia cadamba is expected to be increased along with the high demand of wood products (6.4 million m' in 2020). M...

Full description

Saved in:
Bibliographic Details
Main Author: Norsyuhadah, Binti Rosman
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2017
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/27797/2/NORSYUHADAH.pdf
http://ir.unimas.my/id/eprint/27797/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.27797
record_format eprints
spelling my.unimas.ir.277972024-02-07T03:53:45Z http://ir.unimas.my/id/eprint/27797/ Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions Norsyuhadah, Binti Rosman SB Plant culture Neolamarckia cadamba belongs to Rubiaceae family which is a fast-growing timber species. It is widely used as traditional medicines and suitable for reforestation. Demand for Neolamarckia cadamba is expected to be increased along with the high demand of wood products (6.4 million m' in 2020). Micropropagation technique helps in mass production of many plants species including Neolamarckia cadamba. However, transplanting the plants to ex vitro conditions continue to be the major bottleneck of micropropagation technique. This study was aimed to establish the suitable method for acclimatization of Neolamarckia cadamba. The plantlets were subjected to different acclimatization approaches. There is a control and two different acclimatization approaches (Method A and Method B). Each method has 3 replicate with 3 plantlets for each replicate. A control is whereby the plantlets were planted without being covered by transparent plastic bag, was used as a comparison with Method A and Method B. In Method A and Method B, the plantlets were covered by transparent plastic bag. However, in Method B, the plantlets were directly supplemented with Gamborg B5 liquid medium without vitamin and sucrose. Percentage ofplant survival rate, plant height, plant fresh weight and leaflength were analyzed by using one way ANOVA and Duncan's Multiple Range Test in SPSS Version 22. The experimental design used was Randomized Complete Design (RCD). The results show that there was significant difference in percentage of plant survival rate, plant height, plant fresh weight and leaf length in Method A and Method B compared to controL Yet, there was no significant difference in percentage of plant survival rate in Method A (88.89%) and Method B (88.89%). Hence, it can be concluded that Method A is the suitable method to acclimatize Neolamarckia cadamba as it is more simple method compared to Method B. Universiti Malaysia Sarawak (UNIMAS) 2017 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/27797/2/NORSYUHADAH.pdf Norsyuhadah, Binti Rosman (2017) Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Norsyuhadah, Binti Rosman
Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
description Neolamarckia cadamba belongs to Rubiaceae family which is a fast-growing timber species. It is widely used as traditional medicines and suitable for reforestation. Demand for Neolamarckia cadamba is expected to be increased along with the high demand of wood products (6.4 million m' in 2020). Micropropagation technique helps in mass production of many plants species including Neolamarckia cadamba. However, transplanting the plants to ex vitro conditions continue to be the major bottleneck of micropropagation technique. This study was aimed to establish the suitable method for acclimatization of Neolamarckia cadamba. The plantlets were subjected to different acclimatization approaches. There is a control and two different acclimatization approaches (Method A and Method B). Each method has 3 replicate with 3 plantlets for each replicate. A control is whereby the plantlets were planted without being covered by transparent plastic bag, was used as a comparison with Method A and Method B. In Method A and Method B, the plantlets were covered by transparent plastic bag. However, in Method B, the plantlets were directly supplemented with Gamborg B5 liquid medium without vitamin and sucrose. Percentage ofplant survival rate, plant height, plant fresh weight and leaflength were analyzed by using one way ANOVA and Duncan's Multiple Range Test in SPSS Version 22. The experimental design used was Randomized Complete Design (RCD). The results show that there was significant difference in percentage of plant survival rate, plant height, plant fresh weight and leaf length in Method A and Method B compared to controL Yet, there was no significant difference in percentage of plant survival rate in Method A (88.89%) and Method B (88.89%). Hence, it can be concluded that Method A is the suitable method to acclimatize Neolamarckia cadamba as it is more simple method compared to Method B.
format Final Year Project Report
author Norsyuhadah, Binti Rosman
author_facet Norsyuhadah, Binti Rosman
author_sort Norsyuhadah, Binti Rosman
title Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
title_short Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
title_full Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
title_fullStr Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
title_full_unstemmed Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions
title_sort acclimatization of tissue cultured neolamarckia cadamba plantlets to ex vitro conditions
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2017
url http://ir.unimas.my/id/eprint/27797/2/NORSYUHADAH.pdf
http://ir.unimas.my/id/eprint/27797/
_version_ 1792160650381754368
score 13.211869

Similar Items