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Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.

Amnesic shellfish poisoning (ASP) is caused by contamination of Domoic Acid (DA) that derived from several genera of diatom, including Pseudo-nitzschia spp. In this study, distribution and abundance of Pseudo-nitzschia was determined in two estuarine water in Kuching, i. e. Santubong and Semariang B...

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Main Author: Suriyanti, Su Nyun Pau
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2008
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/27726/2/Suriyanti%20Su%20Nyun%20Pau%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/27726/
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spelling my.unimas.ir.277262024-02-29T06:59:36Z http://ir.unimas.my/id/eprint/27726/ Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy. Suriyanti, Su Nyun Pau SH Aquaculture. Fisheries. Angling Amnesic shellfish poisoning (ASP) is caused by contamination of Domoic Acid (DA) that derived from several genera of diatom, including Pseudo-nitzschia spp. In this study, distribution and abundance of Pseudo-nitzschia was determined in two estuarine water in Kuching, i. e. Santubong and Semariang Batu. Net (20µm mesh size) hauled sample were collected and preserved for identification and live sample were used for clonal culture establishment. Identification of Pseudo-nitzschia was carried out using light microscope (LM) and electron microscope (EM). Our result showed that at least 3 species of Pseudo-nitzschia was found in the water, namely P. fraudulenta, P. subpacifica and one unidentified species. Toxin analysis using AOAC mouse bioassay showed that the extract of P. fraudulenta killed mice within 24 hours. However, further toxin analysis using Iligh Performance Liquid Chromatography (HPLC) or Liquid Chromatography Mass Spectrometry (LCMS) is essential. This represents the first report of potential toxic Pseudo-nitzschia spp. in Malaysian waters. Universiti Malaysia Sarawak (UNIMAS) 2008 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/27726/2/Suriyanti%20Su%20Nyun%20Pau%28fulltext%29.pdf Suriyanti, Su Nyun Pau (2008) Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Suriyanti, Su Nyun Pau
Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
description Amnesic shellfish poisoning (ASP) is caused by contamination of Domoic Acid (DA) that derived from several genera of diatom, including Pseudo-nitzschia spp. In this study, distribution and abundance of Pseudo-nitzschia was determined in two estuarine water in Kuching, i. e. Santubong and Semariang Batu. Net (20µm mesh size) hauled sample were collected and preserved for identification and live sample were used for clonal culture establishment. Identification of Pseudo-nitzschia was carried out using light microscope (LM) and electron microscope (EM). Our result showed that at least 3 species of Pseudo-nitzschia was found in the water, namely P. fraudulenta, P. subpacifica and one unidentified species. Toxin analysis using AOAC mouse bioassay showed that the extract of P. fraudulenta killed mice within 24 hours. However, further toxin analysis using Iligh Performance Liquid Chromatography (HPLC) or Liquid Chromatography Mass Spectrometry (LCMS) is essential. This represents the first report of potential toxic Pseudo-nitzschia spp. in Malaysian waters.
format Final Year Project Report
author Suriyanti, Su Nyun Pau
author_facet Suriyanti, Su Nyun Pau
author_sort Suriyanti, Su Nyun Pau
title Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
title_short Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
title_full Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
title_fullStr Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
title_full_unstemmed Identification of Pseudo-nitzchia spp. ( Bacillariophyceae) at two different locations of Kuching estuarine waters by light and electron microscopy.
title_sort identification of pseudo-nitzchia spp. ( bacillariophyceae) at two different locations of kuching estuarine waters by light and electron microscopy.
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2008
url http://ir.unimas.my/id/eprint/27726/2/Suriyanti%20Su%20Nyun%20Pau%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/27726/
_version_ 1792658553157189632
score 13.211869

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