Integrating an automated sorting machine with image processing device colony enumeration procedure

The automation of colony enumeration procedure has been increasingly popular among researchers worldwide. In the field of microbiology, accurate quantitative analysis is important to determine the quality of a tested sample. The technology of powerful imaging processor for this procedure has been d...

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Bibliographic Details
Main Author: Joel, Anak Ngikoh @ Nyikoh
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS) 2017
Subjects:
Online Access:http://ir.unimas.my/id/eprint/25655/1/Integrating%20an%20automated%20sorting%20machine%2024pgs.pdf
http://ir.unimas.my/id/eprint/25655/4/Integrating%20an%20automated%20sorting%20machine%20ft.pdf
http://ir.unimas.my/id/eprint/25655/
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Summary:The automation of colony enumeration procedure has been increasingly popular among researchers worldwide. In the field of microbiology, accurate quantitative analysis is important to determine the quality of a tested sample. The technology of powerful imaging processor for this procedure has been developed in recent years to help reduce the amount of manual work require in the laboratories. As time progresses the technology is getting more affordable and inexpensive which allow many of these laboratories to start adopting this technology to their system. However, this technology is only limited in processing the image of samples captured on a cultured petri dish. With the increasing amount of petri dishes to be analyzed, it is necessary to develop a sorting machine that can handle a high number of petri dishes. In this research, an automated sorting machine that can be integrated together with the image processing technology for counting bacterial colony-forming units (CFUs) is developed. The purpose of this research is to fabricate and test the mechanism of the prototype of the machine at a relatively low cost. The machine consists of two systems; a carousel storage system where a few stacks of petri dishes can be stacked on top of it and a petri dish handling system to extract the petri dishes from the storage to the imaging area and back to the storage. The mechanism of the proposed design were developed and tested in the Solidwork CAD software before testing were carried out in full scale prototype. Finally, with further improvement on the electronic implementation of the study, this machine should be capable of processing a high number of petri dishes without human intervention.