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Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population

Objective: To determine the polymorphic allele and genotype frequencies of Proprotein Convertase Subtilisin/Kexin type 9 PCSK9 g.24382G > A. It aimed to elucidate the association of the polymorphic allele and genotypes with clinical profiles such as total cholesterol (TC), high- density lipoprote...

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Main Authors: Mohd Aminudin, Mustapha, Sim, Sai-Peng, Helmy, Hazmi, Goh, Maybelline Boon Ling
Format: Article
Language:English
English
Published: Japan International Cultural Exchange Foundation (JICEF) 2019
Subjects:
Q Science (General)
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/25050/2/%E8%A1%A81-4.pdf
http://ir.unimas.my/id/eprint/25050/5/Mohd%20Aminudin.pdf
http://ir.unimas.my/id/eprint/25050/
http://www.seronjihou.co.jp/imj/
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spelling my.unimas.ir.250502021-04-21T00:23:00Z http://ir.unimas.my/id/eprint/25050/ Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population Mohd Aminudin, Mustapha Sim, Sai-Peng Helmy, Hazmi Goh, Maybelline Boon Ling Q Science (General) R Medicine (General) Objective: To determine the polymorphic allele and genotype frequencies of Proprotein Convertase Subtilisin/Kexin type 9 PCSK9 g.24382G > A. It aimed to elucidate the association of the polymorphic allele and genotypes with clinical profiles such as total cholesterol (TC), high- density lipoprotein (HDL), low- density lipoprotein (LDL) and homocysteine level in the Bidayuh ethnic group in Sarawak. Material and Methods: One hundred and thirty-nine (139) individuals from the Bidayuh ethnic group in Sarawak participated as study subjects. The Allele Specific PCR (AS-PCR) was used in the genotyping of PCSK9 g.24382G > A to detect the selected SNPs variant (Polymorphisms). Association of genotype frequencies with clinical profile was calculated using One Way ANOVA. As for the association of allele frequencies with clinical profile, Independent Sample T test was used. Results: Heterozygous genotype was statistically higher in normal level of total cholesterol level, LDL and homocysteine level whereas high level of total cholesterol and HDL ratio was statistically higher in variant genotype compared to homozygous genotype (p < 0.05). Heterozygous and variant genotypes of PCSK9 g.24382G > A show that it is significantly associated with high homocysteine level, F (1,137) = 5.018 (p = 0.027). Conclusion: Our results showed that the genetic diversity of PCSK9 gene influences the susceptibility to increased level of homocysteine in the Malaysian population and also support the involvement of LDLR mediated pathways in the process of Familial Hypercholesterolemia. Japan International Cultural Exchange Foundation (JICEF) 2019-06 Article PeerReviewed text en http://ir.unimas.my/id/eprint/25050/2/%E8%A1%A81-4.pdf text en http://ir.unimas.my/id/eprint/25050/5/Mohd%20Aminudin.pdf Mohd Aminudin, Mustapha and Sim, Sai-Peng and Helmy, Hazmi and Goh, Maybelline Boon Ling (2019) Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population. International Medical Journal, 26 (3). pp. 172-176. ISSN 1341-2051 http://www.seronjihou.co.jp/imj/
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
R Medicine (General)
spellingShingle Q Science (General)
R Medicine (General)
Mohd Aminudin, Mustapha
Sim, Sai-Peng
Helmy, Hazmi
Goh, Maybelline Boon Ling
Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
description Objective: To determine the polymorphic allele and genotype frequencies of Proprotein Convertase Subtilisin/Kexin type 9 PCSK9 g.24382G > A. It aimed to elucidate the association of the polymorphic allele and genotypes with clinical profiles such as total cholesterol (TC), high- density lipoprotein (HDL), low- density lipoprotein (LDL) and homocysteine level in the Bidayuh ethnic group in Sarawak. Material and Methods: One hundred and thirty-nine (139) individuals from the Bidayuh ethnic group in Sarawak participated as study subjects. The Allele Specific PCR (AS-PCR) was used in the genotyping of PCSK9 g.24382G > A to detect the selected SNPs variant (Polymorphisms). Association of genotype frequencies with clinical profile was calculated using One Way ANOVA. As for the association of allele frequencies with clinical profile, Independent Sample T test was used. Results: Heterozygous genotype was statistically higher in normal level of total cholesterol level, LDL and homocysteine level whereas high level of total cholesterol and HDL ratio was statistically higher in variant genotype compared to homozygous genotype (p < 0.05). Heterozygous and variant genotypes of PCSK9 g.24382G > A show that it is significantly associated with high homocysteine level, F (1,137) = 5.018 (p = 0.027). Conclusion: Our results showed that the genetic diversity of PCSK9 gene influences the susceptibility to increased level of homocysteine in the Malaysian population and also support the involvement of LDLR mediated pathways in the process of Familial Hypercholesterolemia.
format Article
author Mohd Aminudin, Mustapha
Sim, Sai-Peng
Helmy, Hazmi
Goh, Maybelline Boon Ling
author_facet Mohd Aminudin, Mustapha
Sim, Sai-Peng
Helmy, Hazmi
Goh, Maybelline Boon Ling
author_sort Mohd Aminudin, Mustapha
title Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
title_short Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
title_full Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
title_fullStr Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
title_full_unstemmed Association of PCSK9 g.24382G > A with Increased Homocysteine Level among Bidayuh Ethnic Group in Sarawak Population
title_sort association of pcsk9 g.24382g > a with increased homocysteine level among bidayuh ethnic group in sarawak population
publisher Japan International Cultural Exchange Foundation (JICEF)
publishDate 2019
url http://ir.unimas.my/id/eprint/25050/2/%E8%A1%A81-4.pdf
http://ir.unimas.my/id/eprint/25050/5/Mohd%20Aminudin.pdf
http://ir.unimas.my/id/eprint/25050/
http://www.seronjihou.co.jp/imj/
_version_ 1698700788953513984
score 13.211869

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