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Effects of enzyme dosage on glucose production from pretreated sago hampas

Nowadays, the depletion of carbon sources likes glucose could be aided by utilizing free waste from sago hampas that contain more than 50% starch. In order to increase the utilization of sago hampas, this study was conducted to determine the effects of spirizyme dosage on glucose production from st...

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Main Author: Muhammad Siddiq, Bin Mohamed Salleh.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2014
Subjects:
Q Science (General)
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/23731/2/Effects%20of%20enzyme%20dosage%20on%20glucose...%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/23731/
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spelling my.unimas.ir.237312023-08-23T03:39:24Z http://ir.unimas.my/id/eprint/23731/ Effects of enzyme dosage on glucose production from pretreated sago hampas Muhammad Siddiq, Bin Mohamed Salleh. Q Science (General) S Agriculture (General) Nowadays, the depletion of carbon sources likes glucose could be aided by utilizing free waste from sago hampas that contain more than 50% starch. In order to increase the utilization of sago hampas, this study was conducted to determine the effects of spirizyme dosage on glucose production from steaming pretreated sago hampas. Three different enzyme dosage were selected which are 0.25 µl/g, 0.5 µl/g, and 1.0 gl/g. The sequential step enzymatic hydrolysis and optimum enzyme loading can be the alternate way to saving the time and the cost of operation. Steaming process which considered as pretreatment stage for further hydrolysis was applied initially before enzymatic hydrolysis. Based on the analysis, the 0.5 µl/g of Spirizyme used is selected as the highest productivity and glucose generated 51.10% (w/w) can be attained at 2 hours only. Interestingly, by using only 0.25 gl/g of Spirizyme the conversion was fairly efficient and as high as 50.19% (w/w). This conversion rate could be considered economically feasible regarding the consumption of enzyme dosage and finally the cost of product. The left fibre treated with celluclast and (3-glucosidase, show the higher production of cellobiose than glucose. The enzyme productivity is less as the glucose released less than 0.2 g/L/h and uneconomical for large scale processing. Universiti Malaysia Sarawak (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/23731/2/Effects%20of%20enzyme%20dosage%20on%20glucose...%28fulltext%29.pdf Muhammad Siddiq, Bin Mohamed Salleh. (2014) Effects of enzyme dosage on glucose production from pretreated sago hampas. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
S Agriculture (General)
spellingShingle Q Science (General)
S Agriculture (General)
Muhammad Siddiq, Bin Mohamed Salleh.
Effects of enzyme dosage on glucose production from pretreated sago hampas
description Nowadays, the depletion of carbon sources likes glucose could be aided by utilizing free waste from sago hampas that contain more than 50% starch. In order to increase the utilization of sago hampas, this study was conducted to determine the effects of spirizyme dosage on glucose production from steaming pretreated sago hampas. Three different enzyme dosage were selected which are 0.25 µl/g, 0.5 µl/g, and 1.0 gl/g. The sequential step enzymatic hydrolysis and optimum enzyme loading can be the alternate way to saving the time and the cost of operation. Steaming process which considered as pretreatment stage for further hydrolysis was applied initially before enzymatic hydrolysis. Based on the analysis, the 0.5 µl/g of Spirizyme used is selected as the highest productivity and glucose generated 51.10% (w/w) can be attained at 2 hours only. Interestingly, by using only 0.25 gl/g of Spirizyme the conversion was fairly efficient and as high as 50.19% (w/w). This conversion rate could be considered economically feasible regarding the consumption of enzyme dosage and finally the cost of product. The left fibre treated with celluclast and (3-glucosidase, show the higher production of cellobiose than glucose. The enzyme productivity is less as the glucose released less than 0.2 g/L/h and uneconomical for large scale processing.
format Final Year Project Report
author Muhammad Siddiq, Bin Mohamed Salleh.
author_facet Muhammad Siddiq, Bin Mohamed Salleh.
author_sort Muhammad Siddiq, Bin Mohamed Salleh.
title Effects of enzyme dosage on glucose production from pretreated sago hampas
title_short Effects of enzyme dosage on glucose production from pretreated sago hampas
title_full Effects of enzyme dosage on glucose production from pretreated sago hampas
title_fullStr Effects of enzyme dosage on glucose production from pretreated sago hampas
title_full_unstemmed Effects of enzyme dosage on glucose production from pretreated sago hampas
title_sort effects of enzyme dosage on glucose production from pretreated sago hampas
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/23731/2/Effects%20of%20enzyme%20dosage%20on%20glucose...%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/23731/
_version_ 1775627236707139584
score 13.211869

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