Staff View: Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling

Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling

This study was undertaken to characterize the antibiotic resistance and randomly amplified polymorphic DNA (RAPD) profiles of Vibrio parahaemolyticus isolates from raw vegetable samples. A total of 46 isolates of V. parahaemolyticus recovered from raw vegetables samples and were confirmed by PCR wer...

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Main Authors:	Tunung, Robin, Jeyaletchumi, P., Noorlis, A., Tang, Y. H., Sandra, A., Ghazali, F. M., Noranizan, Binti Mohd Adzahan, Lesley, Maurice Bilung, Haresh, K. K., Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Son, Radu
Format:	Article
Language:	English
Published:	International Food Research Journal (IFRJ) 2012
Subjects:	Q Science (General) QH301 Biology QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/21739/1/Tunung.pdf http://ir.unimas.my/id/eprint/21739/ http://www.ifrj.upm.edu.my/19%20(02)%202012/(13)IFRJ-2012%20Tunung.pdf
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spelling	my.unimas.ir.217392021-07-01T14:18:42Z http://ir.unimas.my/id/eprint/21739/ Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling Tunung, Robin Jeyaletchumi, P. Noorlis, A. Tang, Y. H. Sandra, A. Ghazali, F. M. Noranizan, Binti Mohd Adzahan Lesley, Maurice Bilung Haresh, K. K. Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Son, Radu Q Science (General) QH301 Biology QR Microbiology This study was undertaken to characterize the antibiotic resistance and randomly amplified polymorphic DNA (RAPD) profiles of Vibrio parahaemolyticus isolates from raw vegetable samples. A total of 46 isolates of V. parahaemolyticus recovered from raw vegetables samples and were confirmed by PCR were analyzed in this study. Most of the isolates were resistant to nalidixic acid (93.48%) and were the least resistant towards imipinem (4.35%). The MAR index results also demonstrated high individual and multiple resistances to antibiotics among the isolates. From the RAPD analysis, the size for RAPD fragments generated ranged from 250 bp to 1,500 bp, with most of the strains contained three major gene fragments of 350, 1,000 and 1,350 bp. The RAPD profiles revealed a high level of DNA sequence diversity within the isolates. Antibiotic resistance and RAPD proved to be effective tools in characterizing and differentiating the V. parahaemolyticus strains International Food Research Journal (IFRJ) 2012 Article PeerReviewed text en http://ir.unimas.my/id/eprint/21739/1/Tunung.pdf Tunung, Robin and Jeyaletchumi, P. and Noorlis, A. and Tang, Y. H. and Sandra, A. and Ghazali, F. M. and Noranizan, Binti Mohd Adzahan and Lesley, Maurice Bilung and Haresh, K. K. and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Son, Radu (2012) Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling. International Food Research Journal, 19 (2). pp. 467-474. ISSN 2231-754 http://www.ifrj.upm.edu.my/19%20(02)%202012/(13)IFRJ-2012%20Tunung.pdf
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) QH301 Biology QR Microbiology
spellingShingle	Q Science (General) QH301 Biology QR Microbiology Tunung, Robin Jeyaletchumi, P. Noorlis, A. Tang, Y. H. Sandra, A. Ghazali, F. M. Noranizan, Binti Mohd Adzahan Lesley, Maurice Bilung Haresh, K. K. Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Son, Radu Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
description	This study was undertaken to characterize the antibiotic resistance and randomly amplified polymorphic DNA (RAPD) profiles of Vibrio parahaemolyticus isolates from raw vegetable samples. A total of 46 isolates of V. parahaemolyticus recovered from raw vegetables samples and were confirmed by PCR were analyzed in this study. Most of the isolates were resistant to nalidixic acid (93.48%) and were the least resistant towards imipinem (4.35%). The MAR index results also demonstrated high individual and multiple resistances to antibiotics among the isolates. From the RAPD analysis, the size for RAPD fragments generated ranged from 250 bp to 1,500 bp, with most of the strains contained three major gene fragments of 350, 1,000 and 1,350 bp. The RAPD profiles revealed a high level of DNA sequence diversity within the isolates. Antibiotic resistance and RAPD proved to be effective tools in characterizing and differentiating the V. parahaemolyticus strains
format	Article
author	Tunung, Robin Jeyaletchumi, P. Noorlis, A. Tang, Y. H. Sandra, A. Ghazali, F. M. Noranizan, Binti Mohd Adzahan Lesley, Maurice Bilung Haresh, K. K. Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Son, Radu
author_facet	Tunung, Robin Jeyaletchumi, P. Noorlis, A. Tang, Y. H. Sandra, A. Ghazali, F. M. Noranizan, Binti Mohd Adzahan Lesley, Maurice Bilung Haresh, K. K. Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Son, Radu
author_sort	Tunung, Robin
title	Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
title_short	Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
title_full	Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
title_fullStr	Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
title_full_unstemmed	Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling
title_sort	biosafety of vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and rapd profiling
publisher	International Food Research Journal (IFRJ)
publishDate	2012
url	http://ir.unimas.my/id/eprint/21739/1/Tunung.pdf http://ir.unimas.my/id/eprint/21739/ http://www.ifrj.upm.edu.my/19%20(02)%202012/(13)IFRJ-2012%20Tunung.pdf
_version_	1705060442798292992
score	13.211869

Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling

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