Cover Image

Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak

Pseudomonas spp. are found to be prevalence in wild animals. Propagation of these opportunistic microorganisms among human and animals became important for public health concern. In this study, Pseudomonas spp. from the feacal, anal, intestine and mouth in wild animals in a conserved forest located...

Full description

Saved in:
Bibliographic Details
Main Author: Choo, Chee Wei.
Format: Final Year Project Report
Language:English
Published: unimas 2014
Subjects:
Q Science (General)
SF Animal culture
Online Access:http://ir.unimas.my/id/eprint/20818/8/Identification%20of%20Pseudomonas%20SPP%20fulltext.pdf
http://ir.unimas.my/id/eprint/20818/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.20818
record_format eprints
spelling my.unimas.ir.208182023-01-30T07:45:36Z http://ir.unimas.my/id/eprint/20818/ Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak Choo, Chee Wei. Q Science (General) SF Animal culture Pseudomonas spp. are found to be prevalence in wild animals. Propagation of these opportunistic microorganisms among human and animals became important for public health concern. In this study, Pseudomonas spp. from the feacal, anal, intestine and mouth in wild animals in a conserved forest located at Saremas Plantation, Miri, Sarawak were identified using biochemical tests and detection of exotoxin A (ETA) gene. Series of conventional biochemical test were done, including gram staining, oxidase test, triple sugar iron test, citrate test, catalase test, macconkey agar test, urease test, growth at 42 ℃ and 4 ℃ and cetrimide agar test to identify the isolates based on enzymatic reactions and metabolic pathways. Through these tests, 12 out of 68 isolates were identified as Pseudomonas spp., including P. aeruginosa, P. chlororaphis, P . cichorii, P. fluorescen, P. putida and other Pseudomonas spp. Five isolates were subjected to detection of 369-bp gene, ETA by using PCR and nonspecific bands were observed. This study suggest that Pseudomonas spp. is prevalent in the mouth and anal sample of wild animals, including snake, birds, and bat unimas 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/20818/8/Identification%20of%20Pseudomonas%20SPP%20fulltext.pdf Choo, Chee Wei. (2014) Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
SF Animal culture
spellingShingle Q Science (General)
SF Animal culture
Choo, Chee Wei.
Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
description Pseudomonas spp. are found to be prevalence in wild animals. Propagation of these opportunistic microorganisms among human and animals became important for public health concern. In this study, Pseudomonas spp. from the feacal, anal, intestine and mouth in wild animals in a conserved forest located at Saremas Plantation, Miri, Sarawak were identified using biochemical tests and detection of exotoxin A (ETA) gene. Series of conventional biochemical test were done, including gram staining, oxidase test, triple sugar iron test, citrate test, catalase test, macconkey agar test, urease test, growth at 42 ℃ and 4 ℃ and cetrimide agar test to identify the isolates based on enzymatic reactions and metabolic pathways. Through these tests, 12 out of 68 isolates were identified as Pseudomonas spp., including P. aeruginosa, P. chlororaphis, P . cichorii, P. fluorescen, P. putida and other Pseudomonas spp. Five isolates were subjected to detection of 369-bp gene, ETA by using PCR and nonspecific bands were observed. This study suggest that Pseudomonas spp. is prevalent in the mouth and anal sample of wild animals, including snake, birds, and bat
format Final Year Project Report
author Choo, Chee Wei.
author_facet Choo, Chee Wei.
author_sort Choo, Chee Wei.
title Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
title_short Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
title_full Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
title_fullStr Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
title_full_unstemmed Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak
title_sort identification of pseudomonas spp. isolated from wild animals in saremas plantation, miri, sarawak
publisher unimas
publishDate 2014
url http://ir.unimas.my/id/eprint/20818/8/Identification%20of%20Pseudomonas%20SPP%20fulltext.pdf
http://ir.unimas.my/id/eprint/20818/
_version_ 1756688303725215744
score 13.211869

Similar Items