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Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago

Metroxylon sagu a mono cot tree native to equatorial swamplands is fast becoming an important source of industrial starch. Sago palm can be grown from seeds but it is generally propagated by suckers. As sago palm cultivation is expanding on a plantation scale, it has become increasingly difficult to...

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Main Author: Rathias, anak Daham
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2006
Subjects:
QK Botany
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/20233/1/Rathias%20ft.pdf
http://ir.unimas.my/id/eprint/20233/
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spelling my.unimas.ir.202332024-06-25T08:03:56Z http://ir.unimas.my/id/eprint/20233/ Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago Rathias, anak Daham QK Botany SB Plant culture Metroxylon sagu a mono cot tree native to equatorial swamplands is fast becoming an important source of industrial starch. Sago palm can be grown from seeds but it is generally propagated by suckers. As sago palm cultivation is expanding on a plantation scale, it has become increasingly difficult to meet the demand for superior planting materials by conventional propagation. A more advance propagating technology has to be developed to produce the sago palm in a short period of time to fulfill the world demand on the sago starch industries. In this research, we are trying to find the best composition of media that able to induce the somatic embryogenesis for the plant regeneration of Metroxylon sagu. The surface of the young lamina explants were sterilized with 3g/1 fungicide for 112 hours, 50mg/l ampicillin for 112 hours, 70% ethanol for 2 minutes and 40% Clorox for 30 minutes. The first media formulation that being tested is MS media with BAP, NAA and 2,4-D combination. There were five different treatments of different concentration ratio being tested under light and dark condition. Out of the five treatments, some treatments which were treatment 1, 4 and 5 showed some positive results where by they able to maintain the explants for about 2-3 weeks. After that, the explants slowly tum brown, dried and died. The second formulation was the MS media with the combination of 2,4-D and 2-iP hormones, 2g/1 charcoal, 4g/l agar, 60mg/l sucrose at pH 5.5. There were seven treatments of different hormones ratios being tested. The experiment was also carried in both light and dark condition. The treatments that were being cultured in dark condition shows some positive results where by it can hold the explants remain axenic for 2 -3 weeks. The explants also look greener for treatment 2, 3, 4 and 6. After that, the cultures slowly tum brown, dried and die. The main factor of failure of this research was the combination of hormones concentration that being used for the media formulation maybe not suitable for sago lamina explants. More researches are needed to find the best media and hormones compositions to induce somatic embryogenesis for plant regeneration from the young sago leaves. Universiti Malaysia Sarawak (UNIMAS) 2006 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/20233/1/Rathias%20ft.pdf Rathias, anak Daham (2006) Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QK Botany
SB Plant culture
spellingShingle QK Botany
SB Plant culture
Rathias, anak Daham
Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
description Metroxylon sagu a mono cot tree native to equatorial swamplands is fast becoming an important source of industrial starch. Sago palm can be grown from seeds but it is generally propagated by suckers. As sago palm cultivation is expanding on a plantation scale, it has become increasingly difficult to meet the demand for superior planting materials by conventional propagation. A more advance propagating technology has to be developed to produce the sago palm in a short period of time to fulfill the world demand on the sago starch industries. In this research, we are trying to find the best composition of media that able to induce the somatic embryogenesis for the plant regeneration of Metroxylon sagu. The surface of the young lamina explants were sterilized with 3g/1 fungicide for 112 hours, 50mg/l ampicillin for 112 hours, 70% ethanol for 2 minutes and 40% Clorox for 30 minutes. The first media formulation that being tested is MS media with BAP, NAA and 2,4-D combination. There were five different treatments of different concentration ratio being tested under light and dark condition. Out of the five treatments, some treatments which were treatment 1, 4 and 5 showed some positive results where by they able to maintain the explants for about 2-3 weeks. After that, the explants slowly tum brown, dried and died. The second formulation was the MS media with the combination of 2,4-D and 2-iP hormones, 2g/1 charcoal, 4g/l agar, 60mg/l sucrose at pH 5.5. There were seven treatments of different hormones ratios being tested. The experiment was also carried in both light and dark condition. The treatments that were being cultured in dark condition shows some positive results where by it can hold the explants remain axenic for 2 -3 weeks. The explants also look greener for treatment 2, 3, 4 and 6. After that, the cultures slowly tum brown, dried and die. The main factor of failure of this research was the combination of hormones concentration that being used for the media formulation maybe not suitable for sago lamina explants. More researches are needed to find the best media and hormones compositions to induce somatic embryogenesis for plant regeneration from the young sago leaves.
format Final Year Project Report
author Rathias, anak Daham
author_facet Rathias, anak Daham
author_sort Rathias, anak Daham
title Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
title_short Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
title_full Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
title_fullStr Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
title_full_unstemmed Development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon Sago
title_sort development of a defined tissue culture media for somac embryogenesis and plant regeneration of metroxylon sago
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/20233/1/Rathias%20ft.pdf
http://ir.unimas.my/id/eprint/20233/
_version_ 1802981824292454400
score 13.211869

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