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Protein profiling of disease piper nigrum

Phytophthora capsid is the main cause of the foot rot disease affected the P. nigrum plant. As the disease can cause economic loss to the agriculture, this research was performed to analyse the existence of any distinct protein between healthy and the diseased plant of the P. nigrum from the roots...

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Main Author: Nur Ezzati, Binti Hamdin.
Format: E-LPTA
Language:English
English
Published: Universiti Malaysia Sarawak (UNIMAS) 2014
Subjects:
QK Botany
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/19998/1/Protein%20profiling%20of%20disease%20piper%20nigrum%20%2824%20pages%29.pdf
http://ir.unimas.my/id/eprint/19998/2/Protein%20profiling%20of%20disease%20piper%20nigrum%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/19998/
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spelling my.unimas.ir.199982018-04-04T03:46:34Z http://ir.unimas.my/id/eprint/19998/ Protein profiling of disease piper nigrum Nur Ezzati, Binti Hamdin. QK Botany SB Plant culture Phytophthora capsid is the main cause of the foot rot disease affected the P. nigrum plant. As the disease can cause economic loss to the agriculture, this research was performed to analyse the existence of any distinct protein between healthy and the diseased plant of the P. nigrum from the roots sample. Two methods of protein extraction were used to identify the difference of protein yield and better quality with quantification of protein using two assays which were Bradford assays and BeA assays. The SDS-PAGE analysis had revealed two different unique proteins band of molecular weight ~25 kDa and ~11 kDa from the diseased samples. Metaproteome has the potential to discover the new proteins contributing to analysis of pathogenesis related proteins. At the end of the experiment, the proteins were successful extracted from the roots of the infected plant with new protein profile discovered showing difference between the diseased and non-diseased P. nigrum. Universiti Malaysia Sarawak (UNIMAS) 2014 E-LPTA NonPeerReviewed text en http://ir.unimas.my/id/eprint/19998/1/Protein%20profiling%20of%20disease%20piper%20nigrum%20%2824%20pages%29.pdf text en http://ir.unimas.my/id/eprint/19998/2/Protein%20profiling%20of%20disease%20piper%20nigrum%20%28fulltext%29.pdf Nur Ezzati, Binti Hamdin. (2014) Protein profiling of disease piper nigrum. [E-LPTA] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QK Botany
SB Plant culture
spellingShingle QK Botany
SB Plant culture
Nur Ezzati, Binti Hamdin.
Protein profiling of disease piper nigrum
description Phytophthora capsid is the main cause of the foot rot disease affected the P. nigrum plant. As the disease can cause economic loss to the agriculture, this research was performed to analyse the existence of any distinct protein between healthy and the diseased plant of the P. nigrum from the roots sample. Two methods of protein extraction were used to identify the difference of protein yield and better quality with quantification of protein using two assays which were Bradford assays and BeA assays. The SDS-PAGE analysis had revealed two different unique proteins band of molecular weight ~25 kDa and ~11 kDa from the diseased samples. Metaproteome has the potential to discover the new proteins contributing to analysis of pathogenesis related proteins. At the end of the experiment, the proteins were successful extracted from the roots of the infected plant with new protein profile discovered showing difference between the diseased and non-diseased P. nigrum.
format E-LPTA
author Nur Ezzati, Binti Hamdin.
author_facet Nur Ezzati, Binti Hamdin.
author_sort Nur Ezzati, Binti Hamdin.
title Protein profiling of disease piper nigrum
title_short Protein profiling of disease piper nigrum
title_full Protein profiling of disease piper nigrum
title_fullStr Protein profiling of disease piper nigrum
title_full_unstemmed Protein profiling of disease piper nigrum
title_sort protein profiling of disease piper nigrum
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/19998/1/Protein%20profiling%20of%20disease%20piper%20nigrum%20%2824%20pages%29.pdf
http://ir.unimas.my/id/eprint/19998/2/Protein%20profiling%20of%20disease%20piper%20nigrum%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/19998/
_version_ 1644513169461215232
score 13.211869

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