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Comparative molecular analysis og re site patterns among members of the human p53 gene family

Thep53 gene family consists of three family members -p53,p63 andp73. Thep53 gene is an established tumour suppressor gene and functions in growth an-est, apoptosis, DNA synthesis and repair, genome stability and cell differentiation. The p63 and p73 genes are involved in growth, development and diff...

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Main Author: Wong, Sook Hooi.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2006
Subjects:
QD Chemistry
Online Access:http://ir.unimas.my/id/eprint/19968/8/Wong%20Sook%20Hooi%20%20fulltext%29.pdf
http://ir.unimas.my/id/eprint/19968/
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spelling my.unimas.ir.199682024-03-08T08:58:20Z http://ir.unimas.my/id/eprint/19968/ Comparative molecular analysis og re site patterns among members of the human p53 gene family Wong, Sook Hooi. QD Chemistry Thep53 gene family consists of three family members -p53,p63 andp73. Thep53 gene is an established tumour suppressor gene and functions in growth an-est, apoptosis, DNA synthesis and repair, genome stability and cell differentiation. The p63 and p73 genes are involved in growth, development and differentiation, whereby the p63 gene is required for limb fon-nation and epidellnis fon-nati on whereas the p73 gene is essential for neurogenesis and pheromone signalling. In this study, p53-gene family members were amplified with RT-PCR using total RNA from bladder tissues. The purifIed RT -PCR products were then subjected to restriction digestion using Haelll, Hin 6I, Hpall, MboI, Pstl, RsaI, SmaJ, TaqI and Tru9I to obtain the RE site patterns for each gene family member. These pat1ems were analyzed and compared for variation assessment. The PCR-RFLP analysis yielded no observable results. Simulation of restriction digestion using MB Advanced DNA Analysis version 6.8 1 so fiwar'e showed that theoretically, the p53 and p73 genes showed higher similarity to each other as compared to with p63 gene, consistent with previous studies. Universiti Malaysia Sarawak (UNIMAS) 2006 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/19968/8/Wong%20Sook%20Hooi%20%20fulltext%29.pdf Wong, Sook Hooi. (2006) Comparative molecular analysis og re site patterns among members of the human p53 gene family. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Wong, Sook Hooi.
Comparative molecular analysis og re site patterns among members of the human p53 gene family
description Thep53 gene family consists of three family members -p53,p63 andp73. Thep53 gene is an established tumour suppressor gene and functions in growth an-est, apoptosis, DNA synthesis and repair, genome stability and cell differentiation. The p63 and p73 genes are involved in growth, development and differentiation, whereby the p63 gene is required for limb fon-nation and epidellnis fon-nati on whereas the p73 gene is essential for neurogenesis and pheromone signalling. In this study, p53-gene family members were amplified with RT-PCR using total RNA from bladder tissues. The purifIed RT -PCR products were then subjected to restriction digestion using Haelll, Hin 6I, Hpall, MboI, Pstl, RsaI, SmaJ, TaqI and Tru9I to obtain the RE site patterns for each gene family member. These pat1ems were analyzed and compared for variation assessment. The PCR-RFLP analysis yielded no observable results. Simulation of restriction digestion using MB Advanced DNA Analysis version 6.8 1 so fiwar'e showed that theoretically, the p53 and p73 genes showed higher similarity to each other as compared to with p63 gene, consistent with previous studies.
format Final Year Project Report
author Wong, Sook Hooi.
author_facet Wong, Sook Hooi.
author_sort Wong, Sook Hooi.
title Comparative molecular analysis og re site patterns among members of the human p53 gene family
title_short Comparative molecular analysis og re site patterns among members of the human p53 gene family
title_full Comparative molecular analysis og re site patterns among members of the human p53 gene family
title_fullStr Comparative molecular analysis og re site patterns among members of the human p53 gene family
title_full_unstemmed Comparative molecular analysis og re site patterns among members of the human p53 gene family
title_sort comparative molecular analysis og re site patterns among members of the human p53 gene family
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/19968/8/Wong%20Sook%20Hooi%20%20fulltext%29.pdf
http://ir.unimas.my/id/eprint/19968/
_version_ 1793163059186892800
score 13.211869

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