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Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production

Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research...

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Main Authors: Nor Hasmaliana, Abdul Manas, Rosli, Md. Illias, Nor Muhammad, Mahadi
Format: Article
Language:English
Published: Taylor and Francis Ltd 2018
Subjects:
TP Chemical technology
Online Access:http://ir.unimas.my/id/eprint/19571/1/Nor%20Hasmaliana.pdf
http://ir.unimas.my/id/eprint/19571/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021921584&doi=10.1080%2f07388551.2017.1339664&partnerID=40&md5=bd34e1c534dc3f09defdd41833eb3055
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spelling my.unimas.ir.195712021-04-28T22:32:10Z http://ir.unimas.my/id/eprint/19571/ Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production Nor Hasmaliana, Abdul Manas Rosli, Md. Illias Nor Muhammad, Mahadi TP Chemical technology Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. © 2017 Informa UK Limited, trading as Taylor & Francis Group. Taylor and Francis Ltd 2018-02-17 Article PeerReviewed text en http://ir.unimas.my/id/eprint/19571/1/Nor%20Hasmaliana.pdf Nor Hasmaliana, Abdul Manas and Rosli, Md. Illias and Nor Muhammad, Mahadi (2018) Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production. Critical Reviews in Biotechnology, 38 (2). pp. 272-293. ISSN 0738-8551 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021921584&doi=10.1080%2f07388551.2017.1339664&partnerID=40&md5=bd34e1c534dc3f09defdd41833eb3055 DOI: 10.1080/07388551.2017.1339664
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nor Hasmaliana, Abdul Manas
Rosli, Md. Illias
Nor Muhammad, Mahadi
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
description Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
format Article
author Nor Hasmaliana, Abdul Manas
Rosli, Md. Illias
Nor Muhammad, Mahadi
author_facet Nor Hasmaliana, Abdul Manas
Rosli, Md. Illias
Nor Muhammad, Mahadi
author_sort Nor Hasmaliana, Abdul Manas
title Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
title_short Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
title_full Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
title_fullStr Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
title_full_unstemmed Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
title_sort strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
publisher Taylor and Francis Ltd
publishDate 2018
url http://ir.unimas.my/id/eprint/19571/1/Nor%20Hasmaliana.pdf
http://ir.unimas.my/id/eprint/19571/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021921584&doi=10.1080%2f07388551.2017.1339664&partnerID=40&md5=bd34e1c534dc3f09defdd41833eb3055
_version_ 1698700770691514368
score 13.211869

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