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Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique

Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen...

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Bibliographic Details
Main Author: Rohana, Binti Muhammad.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2005
Subjects:
QK Botany
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf
http://ir.unimas.my/id/eprint/18905/
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spelling my.unimas.ir.189052024-06-25T08:42:27Z http://ir.unimas.my/id/eprint/18905/ Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique Rohana, Binti Muhammad. QK Botany SB Plant culture Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen two sets of primers designed from rice to look for possible nas in sago. CTAB method for miniprep extraction produced satisfactory results where DNA bands were observed in 1% agarose gel electrophoresis. Once the unpurified DNA was subjected for PCR with two sets of primers, a single band approximately 650bp was observed in one set of primers. Unfortunately, the cloning step could not be proceed due to time constrain. Universiti Malaysia Sarawak (UNIMAS) 2005 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf Rohana, Binti Muhammad. (2005) Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QK Botany
SB Plant culture
spellingShingle QK Botany
SB Plant culture
Rohana, Binti Muhammad.
Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
description Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen two sets of primers designed from rice to look for possible nas in sago. CTAB method for miniprep extraction produced satisfactory results where DNA bands were observed in 1% agarose gel electrophoresis. Once the unpurified DNA was subjected for PCR with two sets of primers, a single band approximately 650bp was observed in one set of primers. Unfortunately, the cloning step could not be proceed due to time constrain.
format Final Year Project Report
author Rohana, Binti Muhammad.
author_facet Rohana, Binti Muhammad.
author_sort Rohana, Binti Muhammad.
title Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
title_short Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
title_full Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
title_fullStr Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
title_full_unstemmed Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
title_sort preliminary screening of nicotianamine synthase (nas) gene in sago palm using pcr technique
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2005
url http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf
http://ir.unimas.my/id/eprint/18905/
_version_ 1802981822460592128
score 13.211869

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