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Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system

Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the AP...

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Main Author: Chung, James Yaw Ming.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2005
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/18074/
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spelling my.unimas.ir.180742023-02-13T08:28:39Z http://ir.unimas.my/id/eprint/18074/ Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system Chung, James Yaw Ming. QR Microbiology Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the APC cDNA fragments from the normal and tumour colorectal specimens are subjected to mutational analysis via restriction enzyme site characterization. Restriction digestions using AluI, HaeIII, Hinfl, HincH, HpaII, MboI, NdeI, RsaI, TaqI, and Trn9I were performed on all eight APC fragments of normal and tumour specimens accordingly. Restriction site map for the gene was constructed whereby assay on APC 2, APC 5, and APC 6 had generated differential restriction site pattern. Tumour APC 2 was showing aberrancy on RsaI, Hinfl, Trn9I, HaeIII, and HpaH; whereas tumour APC 5 was aberrant on AluI, and tumour APC 6 was aberrant on TaqI. This will facilitate the recognition of potential mutation marker to screen for APC aberrancy, allowing the comparative study between APC restriction site pattern of normal and tumour system. Universiti Malaysia Sarawak (UNIMAS) 2005 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf Chung, James Yaw Ming. (2005) Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Chung, James Yaw Ming.
Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
description Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the APC cDNA fragments from the normal and tumour colorectal specimens are subjected to mutational analysis via restriction enzyme site characterization. Restriction digestions using AluI, HaeIII, Hinfl, HincH, HpaII, MboI, NdeI, RsaI, TaqI, and Trn9I were performed on all eight APC fragments of normal and tumour specimens accordingly. Restriction site map for the gene was constructed whereby assay on APC 2, APC 5, and APC 6 had generated differential restriction site pattern. Tumour APC 2 was showing aberrancy on RsaI, Hinfl, Trn9I, HaeIII, and HpaH; whereas tumour APC 5 was aberrant on AluI, and tumour APC 6 was aberrant on TaqI. This will facilitate the recognition of potential mutation marker to screen for APC aberrancy, allowing the comparative study between APC restriction site pattern of normal and tumour system.
format Final Year Project Report
author Chung, James Yaw Ming.
author_facet Chung, James Yaw Ming.
author_sort Chung, James Yaw Ming.
title Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
title_short Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
title_full Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
title_fullStr Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
title_full_unstemmed Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
title_sort restriction site mapping analysis of the adenomatous poliposis coli (apc) gene in human colorectal system
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2005
url http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/18074/
_version_ 1758582506975657984
score 13.211869

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