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Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae

This study concerned about the screening and cloning of amylase and ceHulase genes from Botryodiplodia theobromae. This fungus is found throughout the tropics and subtropics using numerous kinds of disease but especially rots of fruits and root crops during storage. In this study, B. theobromae used...

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Bibliographic Details
Main Author: Elizah, Mohamed.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2004
Subjects:
QK Botany
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/17616/1/Elizah%20Mohamed%20ft.pdf
http://ir.unimas.my/id/eprint/17616/
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spelling my.unimas.ir.176162023-08-10T09:32:42Z http://ir.unimas.my/id/eprint/17616/ Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae Elizah, Mohamed. QK Botany SB Plant culture This study concerned about the screening and cloning of amylase and ceHulase genes from Botryodiplodia theobromae. This fungus is found throughout the tropics and subtropics using numerous kinds of disease but especially rots of fruits and root crops during storage. In this study, B. theobromae used was isolated from mango and cultured on minimal media, minimal media containing starch and minimal media containing carboxymetylcellulose (CMC) as selective media. The existence of these enzymes was indicated by enzyme degradation of the media that produced clear haloes around the culture of fungus. Molecular analysis that have done on this fungus are: genomic DNA extraction, restriction endonuclease digestion. Polymerase Chain Reaction (PCR). cloning and a few confirmation test for clones. In this study. clones of amylase and cellulase and genes were success produced. Universiti Malaysia Sarawak, (UNIMAS) 2004 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/17616/1/Elizah%20Mohamed%20ft.pdf Elizah, Mohamed. (2004) Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QK Botany
SB Plant culture
spellingShingle QK Botany
SB Plant culture
Elizah, Mohamed.
Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
description This study concerned about the screening and cloning of amylase and ceHulase genes from Botryodiplodia theobromae. This fungus is found throughout the tropics and subtropics using numerous kinds of disease but especially rots of fruits and root crops during storage. In this study, B. theobromae used was isolated from mango and cultured on minimal media, minimal media containing starch and minimal media containing carboxymetylcellulose (CMC) as selective media. The existence of these enzymes was indicated by enzyme degradation of the media that produced clear haloes around the culture of fungus. Molecular analysis that have done on this fungus are: genomic DNA extraction, restriction endonuclease digestion. Polymerase Chain Reaction (PCR). cloning and a few confirmation test for clones. In this study. clones of amylase and cellulase and genes were success produced.
format Final Year Project Report
author Elizah, Mohamed.
author_facet Elizah, Mohamed.
author_sort Elizah, Mohamed.
title Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
title_short Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
title_full Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
title_fullStr Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
title_full_unstemmed Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
title_sort screening and cloning of amylase and cellulase genes from botryodiplodia theobromae
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2004
url http://ir.unimas.my/id/eprint/17616/1/Elizah%20Mohamed%20ft.pdf
http://ir.unimas.my/id/eprint/17616/
_version_ 1775627205399805952
score 13.211869

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