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Molecular cloning of partial and full length of human periostin gene into expression construct

Periostin which originally named as the osteoblast-specific factor 2 is highly homologous to jJigH3, a molecule induced by transfonning growth factor (TGF) -131; andfasciclin 1 an insect adhesion molecule. Penostin was proved to play an essential role in promoting the growth of tumor cells. Function...

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Main Author: Aziana, Binti Abu Hassan.
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2006
Subjects:
QD Chemistry
Online Access:http://ir.unimas.my/id/eprint/17582/3/Aziana%20Binti%20Abu%20Hassan%20ft.pdf
http://ir.unimas.my/id/eprint/17582/
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spelling my.unimas.ir.175822023-01-30T07:41:01Z http://ir.unimas.my/id/eprint/17582/ Molecular cloning of partial and full length of human periostin gene into expression construct Aziana, Binti Abu Hassan. QD Chemistry Periostin which originally named as the osteoblast-specific factor 2 is highly homologous to jJigH3, a molecule induced by transfonning growth factor (TGF) -131; andfasciclin 1 an insect adhesion molecule. Penostin was proved to play an essential role in promoting the growth of tumor cells. Functional studies of human periostin should be perfonned to intensively understand the function and expression of periostin in nonnal and cancer tissues. In this report, cDNA fragments of periostin from human nonnal colon total RNA are isolated by R T -PCR for the purpose of amplification of the gene fragments, clone into pTargeT™ Mammalian Expression Vector System and sent to DNA sequencing analysis. Infonnation generated from DNA sequencing analysis is used to confinn the successful construction of the periostin into expression vector. Expression constructed ofperiostin into pTargeT™ vector can be used to begin functional studies of penostin inside mammalian cell lines system. The partial fragment of penostin gene with a predicted size of 685 bp was successfully amplified. However subcloned of periostin in pTargeTTM vector was unsuccessful due to several problems. Therefore, DNA sequencing analysis was not perfonn. Universiti Malaysia Sarawak, (UNIMAS) 2006 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/17582/3/Aziana%20Binti%20Abu%20Hassan%20ft.pdf Aziana, Binti Abu Hassan. (2006) Molecular cloning of partial and full length of human periostin gene into expression construct. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Aziana, Binti Abu Hassan.
Molecular cloning of partial and full length of human periostin gene into expression construct
description Periostin which originally named as the osteoblast-specific factor 2 is highly homologous to jJigH3, a molecule induced by transfonning growth factor (TGF) -131; andfasciclin 1 an insect adhesion molecule. Penostin was proved to play an essential role in promoting the growth of tumor cells. Functional studies of human periostin should be perfonned to intensively understand the function and expression of periostin in nonnal and cancer tissues. In this report, cDNA fragments of periostin from human nonnal colon total RNA are isolated by R T -PCR for the purpose of amplification of the gene fragments, clone into pTargeT™ Mammalian Expression Vector System and sent to DNA sequencing analysis. Infonnation generated from DNA sequencing analysis is used to confinn the successful construction of the periostin into expression vector. Expression constructed ofperiostin into pTargeT™ vector can be used to begin functional studies of penostin inside mammalian cell lines system. The partial fragment of penostin gene with a predicted size of 685 bp was successfully amplified. However subcloned of periostin in pTargeTTM vector was unsuccessful due to several problems. Therefore, DNA sequencing analysis was not perfonn.
format Final Year Project Report
author Aziana, Binti Abu Hassan.
author_facet Aziana, Binti Abu Hassan.
author_sort Aziana, Binti Abu Hassan.
title Molecular cloning of partial and full length of human periostin gene into expression construct
title_short Molecular cloning of partial and full length of human periostin gene into expression construct
title_full Molecular cloning of partial and full length of human periostin gene into expression construct
title_fullStr Molecular cloning of partial and full length of human periostin gene into expression construct
title_full_unstemmed Molecular cloning of partial and full length of human periostin gene into expression construct
title_sort molecular cloning of partial and full length of human periostin gene into expression construct
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/17582/3/Aziana%20Binti%20Abu%20Hassan%20ft.pdf
http://ir.unimas.my/id/eprint/17582/
_version_ 1756688295151009792
score 13.211869

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