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Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings

Pleurotus sajor-caju (Fr.) Sings, a mushroom of the family Pleurotaceae, is gaining popularity due to its high nutrient content and capability of growing on various agricultural wastes. There is a need to breed new strain of P. sajor-caju to meet the rising demands of the increasing human populati...

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Main Authors: Fung, Jessica Lee Ying, Mohamad Hasnul., B, Kuok, Freddy San Yeo
Format: Article
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2017
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/17522/7/Variations.pdf
http://ir.unimas.my/id/eprint/17522/
http://www.bjrst.unimas.my/index.php/online-issue
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spelling my.unimas.ir.175222022-06-20T07:50:32Z http://ir.unimas.my/id/eprint/17522/ Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings Fung, Jessica Lee Ying Mohamad Hasnul., B Kuok, Freddy San Yeo SB Plant culture Pleurotus sajor-caju (Fr.) Sings, a mushroom of the family Pleurotaceae, is gaining popularity due to its high nutrient content and capability of growing on various agricultural wastes. There is a need to breed new strain of P. sajor-caju to meet the rising demands of the increasing human population. Strain improvement is achievable through selection and hybridization. Unfortunately, there is limited information regarding the genetic variations of P. sajor caju in Malaysia. Therefore, this study is of interest to document the morphological variations of single basidiospore isolates and to generate hybrids. A total of 200 single basidiospore isolates (SB) obtained from a commercialized strain of P. sajor-caju were obtained from local supermarket in Kuching, Sarawak, and cultured individually on potato dextrose agar. These 200 SBs were characterized morphologically and divided into three main groups based on colony morphology i.e. scattered, rough and smooth. Variations can still be observed in each main group. From each main group, SBs representing the variations were further categorized based on their colony diameter growth after 7 days of post inoculation (CD-7dpi), i.e. slow growing CD-7dpi (SGCD-7), medium fast growing CD-7dpi (MFGCD-7) and fast growing CD-7dpi (FGCD-7). Ten FGCD-7 and ten SGCD-7 isolates were selected for hybridization. The selected SBs were hybridized in all possible pairings without repetition. Sixteen hybridized isolates were recognized and characterized based on CD-7dpi. For all FGCD-7 pairings, SGCD-7 pairings, and between FGCD-7 and SGCD-7 pairings, hybridized isolates had higher CD-7dpi than at least one of its parents were identified. The new hybridized isolates are interesting materials for future study. Universiti Malaysia Sarawak, (UNIMAS) 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17522/7/Variations.pdf Fung, Jessica Lee Ying and Mohamad Hasnul., B and Kuok, Freddy San Yeo (2017) Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings. Borneo Journal of Resource Science and Technology, 7 (1). pp. 47-55. ISSN 2229-9769 http://www.bjrst.unimas.my/index.php/online-issue
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Fung, Jessica Lee Ying
Mohamad Hasnul., B
Kuok, Freddy San Yeo
Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
description Pleurotus sajor-caju (Fr.) Sings, a mushroom of the family Pleurotaceae, is gaining popularity due to its high nutrient content and capability of growing on various agricultural wastes. There is a need to breed new strain of P. sajor-caju to meet the rising demands of the increasing human population. Strain improvement is achievable through selection and hybridization. Unfortunately, there is limited information regarding the genetic variations of P. sajor caju in Malaysia. Therefore, this study is of interest to document the morphological variations of single basidiospore isolates and to generate hybrids. A total of 200 single basidiospore isolates (SB) obtained from a commercialized strain of P. sajor-caju were obtained from local supermarket in Kuching, Sarawak, and cultured individually on potato dextrose agar. These 200 SBs were characterized morphologically and divided into three main groups based on colony morphology i.e. scattered, rough and smooth. Variations can still be observed in each main group. From each main group, SBs representing the variations were further categorized based on their colony diameter growth after 7 days of post inoculation (CD-7dpi), i.e. slow growing CD-7dpi (SGCD-7), medium fast growing CD-7dpi (MFGCD-7) and fast growing CD-7dpi (FGCD-7). Ten FGCD-7 and ten SGCD-7 isolates were selected for hybridization. The selected SBs were hybridized in all possible pairings without repetition. Sixteen hybridized isolates were recognized and characterized based on CD-7dpi. For all FGCD-7 pairings, SGCD-7 pairings, and between FGCD-7 and SGCD-7 pairings, hybridized isolates had higher CD-7dpi than at least one of its parents were identified. The new hybridized isolates are interesting materials for future study.
format Article
author Fung, Jessica Lee Ying
Mohamad Hasnul., B
Kuok, Freddy San Yeo
author_facet Fung, Jessica Lee Ying
Mohamad Hasnul., B
Kuok, Freddy San Yeo
author_sort Fung, Jessica Lee Ying
title Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
title_short Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
title_full Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
title_fullStr Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
title_full_unstemmed Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
title_sort variations and hybridization compatibility of single basidiospore isolates of pleurotus sajor-caju (fr.) sings
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2017
url http://ir.unimas.my/id/eprint/17522/7/Variations.pdf
http://ir.unimas.my/id/eprint/17522/
http://www.bjrst.unimas.my/index.php/online-issue
_version_ 1736838262619635712
score 13.211869

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