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Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes’ involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines deri...

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Main Authors: Sim, Edmund U. H., Chan, Stella Li-Li, Ng, Kher-Lee, Lee, Choon-Weng, Narayanan, Kumaran
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2016
Subjects:
Q Science (General)
QM Human anatomy
Online Access:http://ir.unimas.my/id/eprint/16906/1/Narayanan.pdf
http://ir.unimas.my/id/eprint/16906/
https://www.researchgate.net/publication/311091456
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spelling my.unimas.ir.169062021-06-09T15:56:44Z http://ir.unimas.my/id/eprint/16906/ Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines Sim, Edmund U. H. Chan, Stella Li-Li Ng, Kher-Lee Lee, Choon-Weng Narayanan, Kumaran Q Science (General) QM Human anatomy Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes’ involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support RPeL27, RPeL41, and RPeL43 as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins. Hindawi Publishing Corporation 2016-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16906/1/Narayanan.pdf Sim, Edmund U. H. and Chan, Stella Li-Li and Ng, Kher-Lee and Lee, Choon-Weng and Narayanan, Kumaran (2016) Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines. Disease Markers, 2016. ISSN 2380-0682 https://www.researchgate.net/publication/311091456 10.1155/2016/5179594
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QM Human anatomy
spellingShingle Q Science (General)
QM Human anatomy
Sim, Edmund U. H.
Chan, Stella Li-Li
Ng, Kher-Lee
Lee, Choon-Weng
Narayanan, Kumaran
Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
description Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes’ involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support RPeL27, RPeL41, and RPeL43 as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins.
format Article
author Sim, Edmund U. H.
Chan, Stella Li-Li
Ng, Kher-Lee
Lee, Choon-Weng
Narayanan, Kumaran
author_facet Sim, Edmund U. H.
Chan, Stella Li-Li
Ng, Kher-Lee
Lee, Choon-Weng
Narayanan, Kumaran
author_sort Sim, Edmund U. H.
title Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
title_short Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
title_full Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
title_fullStr Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
title_full_unstemmed Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines
title_sort human ribosomal proteins rpel27, rpel43, and rpel41 are upregulated in nasopharyngeal carcinoma cell lines
publisher Hindawi Publishing Corporation
publishDate 2016
url http://ir.unimas.my/id/eprint/16906/1/Narayanan.pdf
http://ir.unimas.my/id/eprint/16906/
https://www.researchgate.net/publication/311091456
_version_ 1702173246463410176
score 13.211869

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