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A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases

Presently, the complete genetic mechanisms for the progression of adenoma to carcinoma for colorectal carcinoma (CRC) remain largely unclear. In order to obtain genetic information of this cancer pathway, we searched for differentially expressed genes in tumours of CRC. Gene expression profiles from...

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Main Authors: Ui, Edmund Hang Sim, Bong, Ivyna Pau Ni, Jamal, Rahman, Nadeson, Sukumar, Balraj, Pauline, Tan, S.K., Sagap, I, Rose, I.M., Lim, P.K.M
Format: E-Article
Language:English
Published: Universiti Sains Malaysia 2006
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/16880/1/A%20PRELIMINARY%20STUDY%20OF%20DIFFERENTIALLY%20EXPRESSED%20GENES%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/16880/
https://www.researchgate.net/publication/229091257
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spelling my.unimas.ir.168802017-08-02T02:06:50Z http://ir.unimas.my/id/eprint/16880/ A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases Ui, Edmund Hang Sim Bong, Ivyna Pau Ni Jamal, Rahman Nadeson, Sukumar Balraj, Pauline Tan, S.K. Sagap, I Rose, I.M. Lim, P.K.M Q Science (General) Presently, the complete genetic mechanisms for the progression of adenoma to carcinoma for colorectal carcinoma (CRC) remain largely unclear. In order to obtain genetic information of this cancer pathway, we searched for differentially expressed genes in tumours of CRC. Gene expression profiles from CRC cases were assessed via the DNA microarray system. We report up-regulation and down-regulation of 819 and 98 genes respectively, in the tumours relative to their normal controls. The differential expression patterns of 121 genes were persistent in all tumours. Thirty three of these are ribosomal proteins (RPs) genes. Comparison of the 121 genes with a public domain gene expression database, the Cancer Gene Expression Database (CGEP), revealed 47 genes to be consistently differentially expressed in colorectal tumours. Among these, 22 are RP genes. Among all RP genes identified in this study the over-expression pattern for six of them is consistent with literature. The up-regulation of RP L32 in CRC tumours was demonstrated for the first time in this study and it was also verified via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Non-RP genes worth noting are the tumour susceptibility gene (TSG101) and the 20-kDa myosin light chain (MLC-2). Albeit small sample size (n = 2 for microarray analysis), our preliminary studies revealed many genes that are brought into the context of CRC tumourigenesis for the first time, thus providing new clues to the genetic events during colorectal carcinogenesis. Universiti Sains Malaysia 2006 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/16880/1/A%20PRELIMINARY%20STUDY%20OF%20DIFFERENTIALLY%20EXPRESSED%20GENES%20%28abstract%29.pdf Ui, Edmund Hang Sim and Bong, Ivyna Pau Ni and Jamal, Rahman and Nadeson, Sukumar and Balraj, Pauline and Tan, S.K. and Sagap, I and Rose, I.M. and Lim, P.K.M (2006) A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases. Jurnal Biosains, 17 (1). pp. 19-37. ISSN 0128-4541 https://www.researchgate.net/publication/229091257
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Ui, Edmund Hang Sim
Bong, Ivyna Pau Ni
Jamal, Rahman
Nadeson, Sukumar
Balraj, Pauline
Tan, S.K.
Sagap, I
Rose, I.M.
Lim, P.K.M
A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
description Presently, the complete genetic mechanisms for the progression of adenoma to carcinoma for colorectal carcinoma (CRC) remain largely unclear. In order to obtain genetic information of this cancer pathway, we searched for differentially expressed genes in tumours of CRC. Gene expression profiles from CRC cases were assessed via the DNA microarray system. We report up-regulation and down-regulation of 819 and 98 genes respectively, in the tumours relative to their normal controls. The differential expression patterns of 121 genes were persistent in all tumours. Thirty three of these are ribosomal proteins (RPs) genes. Comparison of the 121 genes with a public domain gene expression database, the Cancer Gene Expression Database (CGEP), revealed 47 genes to be consistently differentially expressed in colorectal tumours. Among these, 22 are RP genes. Among all RP genes identified in this study the over-expression pattern for six of them is consistent with literature. The up-regulation of RP L32 in CRC tumours was demonstrated for the first time in this study and it was also verified via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Non-RP genes worth noting are the tumour susceptibility gene (TSG101) and the 20-kDa myosin light chain (MLC-2). Albeit small sample size (n = 2 for microarray analysis), our preliminary studies revealed many genes that are brought into the context of CRC tumourigenesis for the first time, thus providing new clues to the genetic events during colorectal carcinogenesis.
format E-Article
author Ui, Edmund Hang Sim
Bong, Ivyna Pau Ni
Jamal, Rahman
Nadeson, Sukumar
Balraj, Pauline
Tan, S.K.
Sagap, I
Rose, I.M.
Lim, P.K.M
author_facet Ui, Edmund Hang Sim
Bong, Ivyna Pau Ni
Jamal, Rahman
Nadeson, Sukumar
Balraj, Pauline
Tan, S.K.
Sagap, I
Rose, I.M.
Lim, P.K.M
author_sort Ui, Edmund Hang Sim
title A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
title_short A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
title_full A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
title_fullStr A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
title_full_unstemmed A Preliminary Study Of Differentially Expressed Genes In Malaysian Colorectal Carcinoma Cases
title_sort preliminary study of differentially expressed genes in malaysian colorectal carcinoma cases
publisher Universiti Sains Malaysia
publishDate 2006
url http://ir.unimas.my/id/eprint/16880/1/A%20PRELIMINARY%20STUDY%20OF%20DIFFERENTIALLY%20EXPRESSED%20GENES%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/16880/
https://www.researchgate.net/publication/229091257
_version_ 1644512476846358528
score 13.211869

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