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Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch

A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. Thi...

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Main Authors: Octavio, Carvajal-Zarrabal, Cirilo, Nolasco-Hipolito, Kopli, Bujang, Ayaaki, Ishizaki
Format: E-Article
Language:English
Published: Springer Berlin Heidelberg 2009
Subjects:
QR Microbiology
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/16285/
https://link.springer.com/article/10.1007%2Fs10295-008-0511-x
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spelling my.unimas.ir.162852017-05-17T07:26:53Z http://ir.unimas.my/id/eprint/16285/ Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch Octavio, Carvajal-Zarrabal Cirilo, Nolasco-Hipolito Kopli, Bujang Ayaaki, Ishizaki QR Microbiology S Agriculture (General) A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l-1 h-1 using a cell concentration of 15 g l-1, 30 � C, pH 5.5 and a dilution rate of 1.24 h-1. Adding 10 g l-1 YE and 2 g l-1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity. Springer Berlin Heidelberg 2009 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf Octavio, Carvajal-Zarrabal and Cirilo, Nolasco-Hipolito and Kopli, Bujang and Ayaaki, Ishizaki (2009) Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch. Journal of Industrial Microbiology & Biotechnology, 36 (3). pp. 409-415. ISSN 1476-5535 (online) https://link.springer.com/article/10.1007%2Fs10295-008-0511-x DOI: 10.1007/s10295-008-0511-x
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
S Agriculture (General)
spellingShingle QR Microbiology
S Agriculture (General)
Octavio, Carvajal-Zarrabal
Cirilo, Nolasco-Hipolito
Kopli, Bujang
Ayaaki, Ishizaki
Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
description A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l-1 h-1 using a cell concentration of 15 g l-1, 30 � C, pH 5.5 and a dilution rate of 1.24 h-1. Adding 10 g l-1 YE and 2 g l-1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity.
format E-Article
author Octavio, Carvajal-Zarrabal
Cirilo, Nolasco-Hipolito
Kopli, Bujang
Ayaaki, Ishizaki
author_facet Octavio, Carvajal-Zarrabal
Cirilo, Nolasco-Hipolito
Kopli, Bujang
Ayaaki, Ishizaki
author_sort Octavio, Carvajal-Zarrabal
title Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
title_short Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
title_full Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
title_fullStr Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
title_full_unstemmed Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
title_sort production of nisin z using lactococcus lactis io-1 from hydrolyzed sago starch
publisher Springer Berlin Heidelberg
publishDate 2009
url http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/16285/
https://link.springer.com/article/10.1007%2Fs10295-008-0511-x
_version_ 1644512335408136192
score 13.211869

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