Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo

Human infections with Plasmodium knowlesi have been misdiagnosed by microscopy as Plasmodium malariae due to their morphological similarities. Although microscopy-identified P. malariae cases have been reported in the state of Sarawak (Malaysian Borno) as early as 1952, recent epidemiological stud...

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Main Authors: Lee, Kim-Sung, Cox-Singh, J., Brooke, George, Asmad, Matusop, Balbir, Singh
Format: Article
Language:English
Published: Elsevier Ltd. 2009
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Online Access:http://ir.unimas.my/id/eprint/15813/1/Plasmodium.pdf
http://ir.unimas.my/id/eprint/15813/
https://www.researchgate.net/publication/24270455_Plasmodium_knowlesi_from_archival_blood_films_Further_evidence_that_human_infections_are_widely_distributed_and_not_newly_emergent_in_Malaysian_Borneo
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spelling my.unimas.ir.158132022-01-20T02:28:00Z http://ir.unimas.my/id/eprint/15813/ Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo Lee, Kim-Sung Cox-Singh, J. Brooke, George Asmad, Matusop Balbir, Singh Q Science (General) Human infections with Plasmodium knowlesi have been misdiagnosed by microscopy as Plasmodium malariae due to their morphological similarities. Although microscopy-identified P. malariae cases have been reported in the state of Sarawak (Malaysian Borno) as early as 1952, recent epidemiological studies suggest the absence of indigenous P. malariae infections. The present study aimed to determine the past incidence and distribution of P. knowlesi infections in the state of Sarawak based on archival blood films from patients diagnosed by microscopy as having P. malariae infections. Nested PCR assays were used to identify Plasmodium species in DNA extracted from 47 thick blood films collected in 1996 from patients in seven different divisions throughout the state of Sarawak. Plasmodium knowlesi DNA was detected in 35 (97.2%) of 36 blood films that were positive for Plasmodium DNA, with patients originating from all seven divisions. Only one sample was positive for P. malariae DNA. This study provides further evidence of the widespread distribution of human infections with P. knowlesi in Sarawak and its past occurrence. Taken together with data from previous studies, our findings suggest that P. knowlesi malaria is not a newly emergent disease in humans. Elsevier Ltd. 2009 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15813/1/Plasmodium.pdf Lee, Kim-Sung and Cox-Singh, J. and Brooke, George and Asmad, Matusop and Balbir, Singh (2009) Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo. International Journal for Parasitology, 39. pp. 1125-1128. ISSN 0020-7519 https://www.researchgate.net/publication/24270455_Plasmodium_knowlesi_from_archival_blood_films_Further_evidence_that_human_infections_are_widely_distributed_and_not_newly_emergent_in_Malaysian_Borneo doi:10.1016/j.ijpara.2009.03.003
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Lee, Kim-Sung
Cox-Singh, J.
Brooke, George
Asmad, Matusop
Balbir, Singh
Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
description Human infections with Plasmodium knowlesi have been misdiagnosed by microscopy as Plasmodium malariae due to their morphological similarities. Although microscopy-identified P. malariae cases have been reported in the state of Sarawak (Malaysian Borno) as early as 1952, recent epidemiological studies suggest the absence of indigenous P. malariae infections. The present study aimed to determine the past incidence and distribution of P. knowlesi infections in the state of Sarawak based on archival blood films from patients diagnosed by microscopy as having P. malariae infections. Nested PCR assays were used to identify Plasmodium species in DNA extracted from 47 thick blood films collected in 1996 from patients in seven different divisions throughout the state of Sarawak. Plasmodium knowlesi DNA was detected in 35 (97.2%) of 36 blood films that were positive for Plasmodium DNA, with patients originating from all seven divisions. Only one sample was positive for P. malariae DNA. This study provides further evidence of the widespread distribution of human infections with P. knowlesi in Sarawak and its past occurrence. Taken together with data from previous studies, our findings suggest that P. knowlesi malaria is not a newly emergent disease in humans.
format Article
author Lee, Kim-Sung
Cox-Singh, J.
Brooke, George
Asmad, Matusop
Balbir, Singh
author_facet Lee, Kim-Sung
Cox-Singh, J.
Brooke, George
Asmad, Matusop
Balbir, Singh
author_sort Lee, Kim-Sung
title Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
title_short Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
title_full Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
title_fullStr Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
title_full_unstemmed Plasmodium knowlesi from archival blood films: Further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo
title_sort plasmodium knowlesi from archival blood films: further evidence that human infections are widely distributed and not newly emergent in malaysian borneo
publisher Elsevier Ltd.
publishDate 2009
url http://ir.unimas.my/id/eprint/15813/1/Plasmodium.pdf
http://ir.unimas.my/id/eprint/15813/
https://www.researchgate.net/publication/24270455_Plasmodium_knowlesi_from_archival_blood_films_Further_evidence_that_human_infections_are_widely_distributed_and_not_newly_emergent_in_Malaysian_Borneo
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score 13.211869