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Crude oil bioremediation by indigenous bacteria isolated from oily sludge

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological,biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF),Serratia marcescens (UMAS2SF) and Klebsiella s...

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Main Authors: Azizan, Nur Hafizah, Kasing, Apun, Lesley Maurice, Bilung, Micky, Vincent, Hairul Azman, Roslan, Ahmad, Husaini
Format: Article
Language:English
Published: Penerbit UTM Press 2016
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/15286/1/Nur%20Hafizah.pdf
http://ir.unimas.my/id/eprint/15286/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996598867&doi=10.11113%2fjt.v78.9945&partnerID=40&md5=1c5e8e88aec2cf2f72657b70ad46ec90
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spelling my.unimas.ir.152862022-02-04T07:21:41Z http://ir.unimas.my/id/eprint/15286/ Crude oil bioremediation by indigenous bacteria isolated from oily sludge Azizan, Nur Hafizah Kasing, Apun Lesley Maurice, Bilung Micky, Vincent Hairul Azman, Roslan Ahmad, Husaini QR Microbiology Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological,biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF),Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 - 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 - C24. Among the isolates,UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 - 90% of n- C14 - C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 - 82% and 1.3% degradation,respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK,A1:B2,B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study,the plot amended with A2:B3 showed the highest PAHs degradation,followed by plots A1:B2,A3:B1:C2 and A1:C3 that was assigned as the third,fourth and fifth best in mineralizing PAHs,respectively Penerbit UTM Press 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15286/1/Nur%20Hafizah.pdf Azizan, Nur Hafizah and Kasing, Apun and Lesley Maurice, Bilung and Micky, Vincent and Hairul Azman, Roslan and Ahmad, Husaini (2016) Crude oil bioremediation by indigenous bacteria isolated from oily sludge. Jurnal Teknologi, 78 (11-2). pp. 61-66. ISSN 01279696 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996598867&doi=10.11113%2fjt.v78.9945&partnerID=40&md5=1c5e8e88aec2cf2f72657b70ad46ec90 DOI: 10.11113/jt.v78.9945
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Azizan, Nur Hafizah
Kasing, Apun
Lesley Maurice, Bilung
Micky, Vincent
Hairul Azman, Roslan
Ahmad, Husaini
Crude oil bioremediation by indigenous bacteria isolated from oily sludge
description Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological,biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF),Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 - 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 - C24. Among the isolates,UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 - 90% of n- C14 - C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 - 82% and 1.3% degradation,respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK,A1:B2,B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study,the plot amended with A2:B3 showed the highest PAHs degradation,followed by plots A1:B2,A3:B1:C2 and A1:C3 that was assigned as the third,fourth and fifth best in mineralizing PAHs,respectively
format Article
author Azizan, Nur Hafizah
Kasing, Apun
Lesley Maurice, Bilung
Micky, Vincent
Hairul Azman, Roslan
Ahmad, Husaini
author_facet Azizan, Nur Hafizah
Kasing, Apun
Lesley Maurice, Bilung
Micky, Vincent
Hairul Azman, Roslan
Ahmad, Husaini
author_sort Azizan, Nur Hafizah
title Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_short Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_full Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_fullStr Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_full_unstemmed Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_sort crude oil bioremediation by indigenous bacteria isolated from oily sludge
publisher Penerbit UTM Press
publishDate 2016
url http://ir.unimas.my/id/eprint/15286/1/Nur%20Hafizah.pdf
http://ir.unimas.my/id/eprint/15286/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996598867&doi=10.11113%2fjt.v78.9945&partnerID=40&md5=1c5e8e88aec2cf2f72657b70ad46ec90
_version_ 1724078572143902720
score 13.211869

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