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Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)

Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alk...

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Main Authors: Rizoh, bin Bosorang, Zaini, Assim, Sulaiman, Hanapi, Fatimah, Abang
Format: E-Article
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2016
Subjects:
QD Chemistry
QL Zoology
Online Access:http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/15031/
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spelling my.unimas.ir.150312017-02-22T07:19:30Z http://ir.unimas.my/id/eprint/15031/ Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) Rizoh, bin Bosorang Zaini, Assim Sulaiman, Hanapi Fatimah, Abang QD Chemistry QL Zoology Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alkanes, and dimethyl-branched alkanes, with carbon chain length ranging from C19 to C39.The study presents the significant correlation between the changes pattern in relative abundance of several CHC and development phase in pupae. Further analysis with multiple linear regression indicated that several CHC compounds showed strong correlation to blowfly pupae age, which were then utilized to create a prediction equation for the age estimation. Finally, the application of the age-dependent model had revealed that estimated age correlated significantly with chronological age of samples C. megacephala., y = 0.97x + 0.092, R2 = 0.9698. The study concluded that, CHC have a potential to estimate age of immature C. megacephala, and possibly in other flies species, and might further be used to determine the PMI. Universiti Malaysia Sarawak, (UNIMAS) 2016 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf Rizoh, bin Bosorang and Zaini, Assim and Sulaiman, Hanapi and Fatimah, Abang (2016) Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae). Borneo Journal of Resource Science and Technology, 6 (2). pp. 11-20. ISSN 2229-9769
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
QL Zoology
spellingShingle QD Chemistry
QL Zoology
Rizoh, bin Bosorang
Zaini, Assim
Sulaiman, Hanapi
Fatimah, Abang
Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
description Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alkanes, and dimethyl-branched alkanes, with carbon chain length ranging from C19 to C39.The study presents the significant correlation between the changes pattern in relative abundance of several CHC and development phase in pupae. Further analysis with multiple linear regression indicated that several CHC compounds showed strong correlation to blowfly pupae age, which were then utilized to create a prediction equation for the age estimation. Finally, the application of the age-dependent model had revealed that estimated age correlated significantly with chronological age of samples C. megacephala., y = 0.97x + 0.092, R2 = 0.9698. The study concluded that, CHC have a potential to estimate age of immature C. megacephala, and possibly in other flies species, and might further be used to determine the PMI.
format E-Article
author Rizoh, bin Bosorang
Zaini, Assim
Sulaiman, Hanapi
Fatimah, Abang
author_facet Rizoh, bin Bosorang
Zaini, Assim
Sulaiman, Hanapi
Fatimah, Abang
author_sort Rizoh, bin Bosorang
title Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
title_short Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
title_full Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
title_fullStr Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
title_full_unstemmed Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
title_sort potential use of cuticular hydrocarbons in estimating the age of blowfly pupae chrysomya megacephala (diptera: calliphoridae)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2016
url http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/15031/
_version_ 1644512049529618432
score 13.211869

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