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Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages

Sago palms (Metroxvlon sago Rottb. ) are high in starch and have the potential to sustain the high demand food and biofuel industry. Studies on sago starch synthase are crucial for better understanding of sago starch for development and improvement. Five different extraction buffer, and four prot...

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Main Author: Chin, Ignatius
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2009
Subjects:
Q Science (General)
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/1494/8/2011-09-prChinIfull.pdf
http://ir.unimas.my/id/eprint/1494/
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spelling my.unimas.ir.14942023-02-07T08:30:12Z http://ir.unimas.my/id/eprint/1494/ Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages Chin, Ignatius Q Science (General) S Agriculture (General) Sago palms (Metroxvlon sago Rottb. ) are high in starch and have the potential to sustain the high demand food and biofuel industry. Studies on sago starch synthase are crucial for better understanding of sago starch for development and improvement. Five different extraction buffer, and four protein concentration methods were tested to optimize the extraction of starch synthase prior to starch synthase assay. GBSS buffer (Denyer et a!., 1995) was found to be the best extraction buffer. Ultrafiltration and ammonium sulphate precipitation at 80 %(w/v) were found to be most suitable to concentrate the crude enzyme extracted. Sago trunk of Buhul growth stage was determinded to contain highest protein. Three distinct bands were observed on 9% SDS-PAGE; -4OkD, -3lkD and -14.4kD and several faint bands. However, the starch synthase assay was unable to be conducter due incomplete materials. Universiti Malaysia Sarawak, (UNIMAS) 2009 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/1494/8/2011-09-prChinIfull.pdf Chin, Ignatius (2009) Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
S Agriculture (General)
spellingShingle Q Science (General)
S Agriculture (General)
Chin, Ignatius
Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
description Sago palms (Metroxvlon sago Rottb. ) are high in starch and have the potential to sustain the high demand food and biofuel industry. Studies on sago starch synthase are crucial for better understanding of sago starch for development and improvement. Five different extraction buffer, and four protein concentration methods were tested to optimize the extraction of starch synthase prior to starch synthase assay. GBSS buffer (Denyer et a!., 1995) was found to be the best extraction buffer. Ultrafiltration and ammonium sulphate precipitation at 80 %(w/v) were found to be most suitable to concentrate the crude enzyme extracted. Sago trunk of Buhul growth stage was determinded to contain highest protein. Three distinct bands were observed on 9% SDS-PAGE; -4OkD, -3lkD and -14.4kD and several faint bands. However, the starch synthase assay was unable to be conducter due incomplete materials.
format Final Year Project Report
author Chin, Ignatius
author_facet Chin, Ignatius
author_sort Chin, Ignatius
title Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
title_short Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
title_full Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
title_fullStr Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
title_full_unstemmed Starch Synthase Analysis Of Sago Palm (Metroxylon Sagu Rottb.) Different Growth Stages
title_sort starch synthase analysis of sago palm (metroxylon sagu rottb.) different growth stages
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2009
url http://ir.unimas.my/id/eprint/1494/8/2011-09-prChinIfull.pdf
http://ir.unimas.my/id/eprint/1494/
_version_ 1758582457727188992
score 13.211869

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