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An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer

Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a bro...

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Main Authors: Wei-Seng, Ho, Kit-Siong, Liew, Shek-Ling, Pang
Format: E-Article
Language:English
Published: IJSTR 2016
Subjects:
GE Environmental Sciences
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/14331/
http://www.ijstr.org/research-paper-publishing.php?month=sep2016
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spelling my.unimas.ir.143312016-11-18T01:26:14Z http://ir.unimas.my/id/eprint/14331/ An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer Wei-Seng, Ho Kit-Siong, Liew Shek-Ling, Pang GE Environmental Sciences Q Science (General) Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Many DNA isolation protocols are available for various plant tissues; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber tree species. A CTAB based protocol has been optimized for isolating genomic DNA from camphor containing timber tree species. Key steps include: 1) using 1% β-mercaptoethanol and 2% PVP 40 (Mr 40,000) in the extraction buffer; 2) sample incubation time, 40 minutes at 65°C, and 3) DNA precipitation at room temperature (25°C). The isolated DNA pellet was transparent colour and the purified genomic DNA is suitable for PCR amplification. Wei-Seng Ho, Kit-Siong Liew, Shek-Ling Pang IJSTR 2016 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf Wei-Seng, Ho and Kit-Siong, Liew and Shek-Ling, Pang (2016) An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer. International Journal Of Scientific & Technology Research, 5 (9). pp. 220-223. ISSN 2277-8616 http://www.ijstr.org/research-paper-publishing.php?month=sep2016
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
Q Science (General)
spellingShingle GE Environmental Sciences
Q Science (General)
Wei-Seng, Ho
Kit-Siong, Liew
Shek-Ling, Pang
An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
description Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Many DNA isolation protocols are available for various plant tissues; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber tree species. A CTAB based protocol has been optimized for isolating genomic DNA from camphor containing timber tree species. Key steps include: 1) using 1% β-mercaptoethanol and 2% PVP 40 (Mr 40,000) in the extraction buffer; 2) sample incubation time, 40 minutes at 65°C, and 3) DNA precipitation at room temperature (25°C). The isolated DNA pellet was transparent colour and the purified genomic DNA is suitable for PCR amplification. Wei-Seng Ho, Kit-Siong Liew, Shek-Ling Pang
format E-Article
author Wei-Seng, Ho
Kit-Siong, Liew
Shek-Ling, Pang
author_facet Wei-Seng, Ho
Kit-Siong, Liew
Shek-Ling, Pang
author_sort Wei-Seng, Ho
title An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
title_short An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
title_full An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
title_fullStr An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
title_full_unstemmed An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
title_sort optimized dna extraction protocol for isolation of high quality genomic dna from camphor containing timber tree species, dryobalanops beccarii dyer
publisher IJSTR
publishDate 2016
url http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/14331/
http://www.ijstr.org/research-paper-publishing.php?month=sep2016
_version_ 1644511884374704128
score 13.211869

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