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Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)

Atotal of twenty endophytic fungi successfully isolated fromMelastomamalabathricum(Senduduk) were examined for their ability to decolourise azo dyes: Congo red, Orange G, andMethyl red and an anthraquinone dye, Remazol Brilliant Blue R. Initial screening on the glucose minimal media agar plates am...

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Main Authors: Ngui, Sing Ngieng, Azham, Zulkharnain, Hairul Azman, Roslan, Ahmad, Husaini
Format: E-Article
Language:English
Published: Hindawi Publishing Corporation 2013
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/13575/1/Decolourisation%20of%20Synthetic%20Dyes%20by%20Endophytic%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/13575/
https://www.hindawi.com/journals/isrn/2013/260730/
http://dx.doi.org/10.5402/2013/260730
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spelling my.unimas.ir.135752016-09-22T23:56:41Z http://ir.unimas.my/id/eprint/13575/ Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum) Ngui, Sing Ngieng Azham, Zulkharnain Hairul Azman, Roslan Ahmad, Husaini SB Plant culture Atotal of twenty endophytic fungi successfully isolated fromMelastomamalabathricum(Senduduk) were examined for their ability to decolourise azo dyes: Congo red, Orange G, andMethyl red and an anthraquinone dye, Remazol Brilliant Blue R. Initial screening on the glucose minimal media agar plates amended with 200mg L−1 of each respective dye showed that only isolate MS8 was able to decolourise all of the four dyes. The isolate decolourised completely both the RBBR and Orange G in the agar medium within 8 days. Further quantitative analysis of the dye decolourisation by isolate MS8 in aqueous minimal medium showed that isolate MS8 was able to decolourise all the tested dyes at varying levels. Dye decolourisation by the isolate MS8 was determined to be 97% for RBBR, 33% for Orange G, 48% for Congo red, and 56% for Methyl red, respectively, within a period of 16 days. Molecular identification of the fungal isolate MS8 using primer ITS1 and ITS4 showed that isolate MS8 shared 99% sequence similarity with Marasmius cladophyllus, a Basidiomycete. The ability to decolourise different types of dyes by isolateMS8 thus suggested a possible application of this fungus in the decolourisation of dyestuff effluents. Hindawi Publishing Corporation 2013 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/13575/1/Decolourisation%20of%20Synthetic%20Dyes%20by%20Endophytic%20%28abstract%29.pdf Ngui, Sing Ngieng and Azham, Zulkharnain and Hairul Azman, Roslan and Ahmad, Husaini (2013) Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum). ISRN Biotechnology, 2013. ISSN 2090-9403 https://www.hindawi.com/journals/isrn/2013/260730/ http://dx.doi.org/10.5402/2013/260730
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Ngui, Sing Ngieng
Azham, Zulkharnain
Hairul Azman, Roslan
Ahmad, Husaini
Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
description Atotal of twenty endophytic fungi successfully isolated fromMelastomamalabathricum(Senduduk) were examined for their ability to decolourise azo dyes: Congo red, Orange G, andMethyl red and an anthraquinone dye, Remazol Brilliant Blue R. Initial screening on the glucose minimal media agar plates amended with 200mg L−1 of each respective dye showed that only isolate MS8 was able to decolourise all of the four dyes. The isolate decolourised completely both the RBBR and Orange G in the agar medium within 8 days. Further quantitative analysis of the dye decolourisation by isolate MS8 in aqueous minimal medium showed that isolate MS8 was able to decolourise all the tested dyes at varying levels. Dye decolourisation by the isolate MS8 was determined to be 97% for RBBR, 33% for Orange G, 48% for Congo red, and 56% for Methyl red, respectively, within a period of 16 days. Molecular identification of the fungal isolate MS8 using primer ITS1 and ITS4 showed that isolate MS8 shared 99% sequence similarity with Marasmius cladophyllus, a Basidiomycete. The ability to decolourise different types of dyes by isolateMS8 thus suggested a possible application of this fungus in the decolourisation of dyestuff effluents.
format E-Article
author Ngui, Sing Ngieng
Azham, Zulkharnain
Hairul Azman, Roslan
Ahmad, Husaini
author_facet Ngui, Sing Ngieng
Azham, Zulkharnain
Hairul Azman, Roslan
Ahmad, Husaini
author_sort Ngui, Sing Ngieng
title Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
title_short Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
title_full Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
title_fullStr Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
title_full_unstemmed Decolourisation of Synthetic Dyes by Endophytic Fungal Flora Isolated from Senduduk Plant (Melastoma malabathricum)
title_sort decolourisation of synthetic dyes by endophytic fungal flora isolated from senduduk plant (melastoma malabathricum)
publisher Hindawi Publishing Corporation
publishDate 2013
url http://ir.unimas.my/id/eprint/13575/1/Decolourisation%20of%20Synthetic%20Dyes%20by%20Endophytic%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/13575/
https://www.hindawi.com/journals/isrn/2013/260730/
http://dx.doi.org/10.5402/2013/260730
_version_ 1644511688655896576
score 13.211869

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