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Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak

The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching, Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the six month study period, 19 samples were collected monthly from seven rivers, followed by simultaneous detec...

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Main Authors: Micky, Vincent, Lawrance, Tuah, Chan, Christy Sien Wei, Lesley Maurice, Bilung, Kasing, Apun
Format: E-Article
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
GE Environmental Sciences
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/12880/1/Application%20of%20the%20Multiplex%20Polymerase%20Chain%20Reaction%20%28M-PCR%29%20for%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/12880/
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spelling my.unimas.ir.128802016-08-10T20:23:23Z http://ir.unimas.my/id/eprint/12880/ Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak Micky, Vincent Lawrance, Tuah Chan, Christy Sien Wei Lesley Maurice, Bilung Kasing, Apun GE Environmental Sciences QR Microbiology The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching, Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the six month study period, 19 samples were collected monthly from seven rivers, followed by simultaneous detection of three Vibrio spp., Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus, in a single tube PCR reaction. Three sets of primers targeting the thermolabile (tl), outer membrane protein (ompW) and hemolysin/cytolysin genes (vulCulsl) of V. parahaemolyticus, V. cholerae and V. vulnificus, respectively, were used. The results indicated that V. parahaemolyticus was the predominant species, occurring approximately 60.9% throughout the sampling period, followed by V. cholerae (23.1%) and V. vulnificus (16.0%). The months of July and December were found to be the months where all three Vibrio spp. were found to be at higher frequencies in the river samples. Results analyzed also indicated that the rivers with the highest prevalence of the three Vibrio spp. were Tambak Sejingkat, followed by Sungai Jernang and Sungai Tabuan. We conclude that m-PCR is a powerful and useful tool for the rapid and simultaneous detection of V. parahaemolyticus, V. cholerae and V. vulnificus from the riverine environments without the need for isolation and culturing. Furthermore, this method is highly specific, and could be applied in diagnostic laboratories for larger scale epidemiological investigations of Vibrio spp. Universiti Malaysia Sarawak, (UNIMAS) 2015 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/12880/1/Application%20of%20the%20Multiplex%20Polymerase%20Chain%20Reaction%20%28M-PCR%29%20for%20%28abstract%29.pdf Micky, Vincent and Lawrance, Tuah and Chan, Christy Sien Wei and Lesley Maurice, Bilung and Kasing, Apun (2015) Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak. Borneo Journal of Resource Science and Technology, 5 (2). pp. 16-27. ISSN 229-9769
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
QR Microbiology
spellingShingle GE Environmental Sciences
QR Microbiology
Micky, Vincent
Lawrance, Tuah
Chan, Christy Sien Wei
Lesley Maurice, Bilung
Kasing, Apun
Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
description The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching, Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the six month study period, 19 samples were collected monthly from seven rivers, followed by simultaneous detection of three Vibrio spp., Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus, in a single tube PCR reaction. Three sets of primers targeting the thermolabile (tl), outer membrane protein (ompW) and hemolysin/cytolysin genes (vulCulsl) of V. parahaemolyticus, V. cholerae and V. vulnificus, respectively, were used. The results indicated that V. parahaemolyticus was the predominant species, occurring approximately 60.9% throughout the sampling period, followed by V. cholerae (23.1%) and V. vulnificus (16.0%). The months of July and December were found to be the months where all three Vibrio spp. were found to be at higher frequencies in the river samples. Results analyzed also indicated that the rivers with the highest prevalence of the three Vibrio spp. were Tambak Sejingkat, followed by Sungai Jernang and Sungai Tabuan. We conclude that m-PCR is a powerful and useful tool for the rapid and simultaneous detection of V. parahaemolyticus, V. cholerae and V. vulnificus from the riverine environments without the need for isolation and culturing. Furthermore, this method is highly specific, and could be applied in diagnostic laboratories for larger scale epidemiological investigations of Vibrio spp.
format E-Article
author Micky, Vincent
Lawrance, Tuah
Chan, Christy Sien Wei
Lesley Maurice, Bilung
Kasing, Apun
author_facet Micky, Vincent
Lawrance, Tuah
Chan, Christy Sien Wei
Lesley Maurice, Bilung
Kasing, Apun
author_sort Micky, Vincent
title Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
title_short Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
title_full Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
title_fullStr Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
title_full_unstemmed Application of the Multiplex Polymerase Chain Reaction (M-PCR) for the Screening of Vibrio spp. from Rivers in Kuching, Sarawak
title_sort application of the multiplex polymerase chain reaction (m-pcr) for the screening of vibrio spp. from rivers in kuching, sarawak
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/12880/1/Application%20of%20the%20Multiplex%20Polymerase%20Chain%20Reaction%20%28M-PCR%29%20for%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/12880/
_version_ 1644511527034683392
score 13.211869

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