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The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer

Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of...

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Main Authors: Mohd Shafi’i, Mohd Suzairi, Tan, Shing Cheng, Abdul Aziz, Ahmad Aizat, Mustapha, Mohd Aminudin, Siti Nurfatimah, Mohd Shahpudin, Zakaria Dzulkarnaen, Andee, Ravindran, Ankathil
Format: Article
Language:English
Published: Elsevier Ltd. 2013
Subjects:
RC Internal medicine
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/11981/
http://www.sciencedirect.com/science/article/pii/S1877782113000945
http://dx.doi.org/10.1016/j.canep.2013.05.007
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spelling my.unimas.ir.119812023-05-29T01:10:43Z http://ir.unimas.my/id/eprint/11981/ The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer Mohd Shafi’i, Mohd Suzairi Tan, Shing Cheng Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Zakaria Dzulkarnaen, Andee Ravindran, Ankathil RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKB1 −94 ins/del ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous (del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR = 2.42, 95% CI = 1.24–4.73, P < 0.01). Conclusions: The variant allele of NFKB1 −94 ins/del ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population. Elsevier Ltd. 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf Mohd Shafi’i, Mohd Suzairi and Tan, Shing Cheng and Abdul Aziz, Ahmad Aizat and Mustapha, Mohd Aminudin and Siti Nurfatimah, Mohd Shahpudin and Zakaria Dzulkarnaen, Andee and Ravindran, Ankathil (2013) The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer. Cancer Epidemiology, 37. pp. 634-638. ISSN 1877-7821 http://www.sciencedirect.com/science/article/pii/S1877782113000945 http://dx.doi.org/10.1016/j.canep.2013.05.007
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic RC Internal medicine
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle RC Internal medicine
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Mohd Shafi’i, Mohd Suzairi
Tan, Shing Cheng
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Zakaria Dzulkarnaen, Andee
Ravindran, Ankathil
The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
description Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKB1 −94 ins/del ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous (del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR = 2.42, 95% CI = 1.24–4.73, P < 0.01). Conclusions: The variant allele of NFKB1 −94 ins/del ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population.
format Article
author Mohd Shafi’i, Mohd Suzairi
Tan, Shing Cheng
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Zakaria Dzulkarnaen, Andee
Ravindran, Ankathil
author_facet Mohd Shafi’i, Mohd Suzairi
Tan, Shing Cheng
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Zakaria Dzulkarnaen, Andee
Ravindran, Ankathil
author_sort Mohd Shafi’i, Mohd Suzairi
title The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
title_short The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
title_full The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
title_fullStr The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
title_full_unstemmed The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
title_sort functional −94 insertion/deletion attg polymorphism in the promoter region of nfkb1 gene increases the risk of sporadic colorectal cancer
publisher Elsevier Ltd.
publishDate 2013
url http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/11981/
http://www.sciencedirect.com/science/article/pii/S1877782113000945
http://dx.doi.org/10.1016/j.canep.2013.05.007
_version_ 1767209790966071296
score 13.211869

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