Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment,...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pelagia Research
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/1170/1/Detection.pdf http://ir.unimas.my/id/eprint/1170/ http://www.pelagiaresearchlibrary.com |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The
species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including
aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a
risk of potential transfer of resistance from aquaculture bacteria to human and animal pathogens. This study was
conducted to isolate P. aeruginosa from fish, prawn and water samples, followed by PCR detection of oprL gene
locus. The antibiotic resistance pattern of the isolates was also determined. Based on the results from PCR analysis
performed, 13 isolates of P. aeruginosawere isolated. All of the isolates tested were resistance to at least one
antibiotic. Highest level of resistance was observed against ampicillin and erythromycin while the lowest was
observed against gentamicin, norfloxacin and nalidixic acid. This study suggested that the presence of the bacteria
in the aquaculture environment may pose the risk of antibiotic resistance to those who are exposed to the
aquaculture environment.Based on the results of this study, it can be said that gentamicin, norflaxin and nalidixic
acid can be effective agents for the treatment of P. aeruginosa. |
|---|