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Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate

This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase assays showed that UMAS P2...

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Main Author: Muhamad Azharuddin, Muhamad Nor
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
GE Environmental Sciences
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf
http://ir.unimas.my/id/eprint/10782/
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spelling my.unimas.ir.107822023-08-18T09:06:22Z http://ir.unimas.my/id/eprint/10782/ Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate Muhamad Azharuddin, Muhamad Nor GE Environmental Sciences S Agriculture (General) This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase assays showed that UMAS P2 andUMAS B2 were able to produce phenolic compound which is ferulic acid. The morphological characteristic and molecular identification was done and the result showed that UMAS P2 is Rhizomucor variabilis and UMAS B2 is Fusarium chlamydosporum. Determination of the production of phenolic compound using Rhizomucor variabilis and Fusarium chlamydosporum under solid state fermentation is achieved by total phenolic content, 'while feruloyl esterase assays for the detection of fem lic acid. The production of phenolic compound by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 0.087 mglg and 0.156 mglg, respectively, while the production of phenolic compound by Fusarium ch/amydosporum under solid state fermentation of rice husk and empty fruit bunch was 0.078 mglg and 0.065 mglg respectively. Besides, the feruloyl esterase activity by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 1.721 U/g and 0.597 U/g, while the feruloyl esterase activity by Fusarium chlamydosporum under solid state fermentation of rice husk and empty fruit bunch was 1.806 U/g and 1.024 U/g, respectively. In conclusion, Rhizomucor variabilis and Fusarium chlamydosporum have an ability to produce phenolic compound by utilizing agricultural waste such as rice husk and empty fruit bunch as carbon source. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf Muhamad Azharuddin, Muhamad Nor (2015) Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
S Agriculture (General)
spellingShingle GE Environmental Sciences
S Agriculture (General)
Muhamad Azharuddin, Muhamad Nor
Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
description This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase assays showed that UMAS P2 andUMAS B2 were able to produce phenolic compound which is ferulic acid. The morphological characteristic and molecular identification was done and the result showed that UMAS P2 is Rhizomucor variabilis and UMAS B2 is Fusarium chlamydosporum. Determination of the production of phenolic compound using Rhizomucor variabilis and Fusarium chlamydosporum under solid state fermentation is achieved by total phenolic content, 'while feruloyl esterase assays for the detection of fem lic acid. The production of phenolic compound by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 0.087 mglg and 0.156 mglg, respectively, while the production of phenolic compound by Fusarium ch/amydosporum under solid state fermentation of rice husk and empty fruit bunch was 0.078 mglg and 0.065 mglg respectively. Besides, the feruloyl esterase activity by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 1.721 U/g and 0.597 U/g, while the feruloyl esterase activity by Fusarium chlamydosporum under solid state fermentation of rice husk and empty fruit bunch was 1.806 U/g and 1.024 U/g, respectively. In conclusion, Rhizomucor variabilis and Fusarium chlamydosporum have an ability to produce phenolic compound by utilizing agricultural waste such as rice husk and empty fruit bunch as carbon source.
format Final Year Project Report
author Muhamad Azharuddin, Muhamad Nor
author_facet Muhamad Azharuddin, Muhamad Nor
author_sort Muhamad Azharuddin, Muhamad Nor
title Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
title_short Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
title_full Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
title_fullStr Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
title_full_unstemmed Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
title_sort screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf
http://ir.unimas.my/id/eprint/10782/
_version_ 1775627177198354432
score 13.211869

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