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Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development

Proline-rich EVHlligand 1 (Prell) and Proline-rich EVHI ligand 2 (PreI2) belong to Prel gene family. Both genes are homologous and participate in cellular integrin activation. Pre12 interacts with GTPase called Ras in GTP-bound state and is essential in regulating cell adhesion and migration. It has...

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Main Author: Jonathan, Chan Chia Jui
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
GE Environmental Sciences
GN Anthropology
Online Access:http://ir.unimas.my/id/eprint/10772/4/Jonathan%20full.pdf
http://ir.unimas.my/id/eprint/10772/
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spelling my.unimas.ir.107722023-06-27T08:16:49Z http://ir.unimas.my/id/eprint/10772/ Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development Jonathan, Chan Chia Jui GE Environmental Sciences GN Anthropology Proline-rich EVHlligand 1 (Prell) and Proline-rich EVHI ligand 2 (PreI2) belong to Prel gene family. Both genes are homologous and participate in cellular integrin activation. Pre12 interacts with GTPase called Ras in GTP-bound state and is essential in regulating cell adhesion and migration. It has been identified to involve in mechanism assisting endocytosis of epidermal growth factor receptor (EGFR) that participates in development of arious diseases such as cancer. However, the comprehensive and mechanistic model of protein signaling involving cell migration is still unavailable. Hence, Prel2 is selected as candidate gene with zebrafish (Danio rerio) in this study to examine its function. This tudy was done partly to verify the occurrence ofPrel2 gene duplication as well as to fulfill our understanding on the importance of Prel2 during vertebrate development. The methodology involves colony peR, plasmid extraction, DNA sequencing, zebrafish cultivation, RNA probe preparation, in situ hybridization, embryo mounting and microscopy. Through these experiments, Prel2 genes were characterized and plasmid with the gene construct was isolated, purified, and ready for RNA probe synthesis for in situ hybridization of zebrafish. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10772/4/Jonathan%20full.pdf Jonathan, Chan Chia Jui (2015) Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
GN Anthropology
spellingShingle GE Environmental Sciences
GN Anthropology
Jonathan, Chan Chia Jui
Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
description Proline-rich EVHlligand 1 (Prell) and Proline-rich EVHI ligand 2 (PreI2) belong to Prel gene family. Both genes are homologous and participate in cellular integrin activation. Pre12 interacts with GTPase called Ras in GTP-bound state and is essential in regulating cell adhesion and migration. It has been identified to involve in mechanism assisting endocytosis of epidermal growth factor receptor (EGFR) that participates in development of arious diseases such as cancer. However, the comprehensive and mechanistic model of protein signaling involving cell migration is still unavailable. Hence, Prel2 is selected as candidate gene with zebrafish (Danio rerio) in this study to examine its function. This tudy was done partly to verify the occurrence ofPrel2 gene duplication as well as to fulfill our understanding on the importance of Prel2 during vertebrate development. The methodology involves colony peR, plasmid extraction, DNA sequencing, zebrafish cultivation, RNA probe preparation, in situ hybridization, embryo mounting and microscopy. Through these experiments, Prel2 genes were characterized and plasmid with the gene construct was isolated, purified, and ready for RNA probe synthesis for in situ hybridization of zebrafish.
format Final Year Project Report
author Jonathan, Chan Chia Jui
author_facet Jonathan, Chan Chia Jui
author_sort Jonathan, Chan Chia Jui
title Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
title_short Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
title_full Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
title_fullStr Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
title_full_unstemmed Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development
title_sort characterization and expression study of prel2a and prel2b on early vetebrate development
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10772/4/Jonathan%20full.pdf
http://ir.unimas.my/id/eprint/10772/
_version_ 1770555372376948736
score 13.211869

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