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Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production

Abundance of lignocellulosic biomass provides a good solution to the demands of energy crops in producing biofuel like biodiesel and bioethanol. In this study, bioethanol was produced from sago hampas via the Simultaneous co-Saccharification and Fermentation (Sc-SF) process, at 2.5% and 5.0% (w/v)...

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Main Authors: Micky, Vincent, Empina, Jabang, Norizawati, Muhamad Nur, Ennry, Esut, Leo, Bulin Unting, Dayang Salwani, Awang Adeni
Format: Article
Language:English
Published: International Commission of Agricultural and Biosystems Engineering 2015
Subjects:
GE Environmental Sciences
Online Access:http://ir.unimas.my/id/eprint/10732/1/Micky.pdf
http://ir.unimas.my/id/eprint/10732/
http://www.cigrjournal.org
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spelling my.unimas.ir.107322022-01-28T02:24:19Z http://ir.unimas.my/id/eprint/10732/ Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production Micky, Vincent Empina, Jabang Norizawati, Muhamad Nur Ennry, Esut Leo, Bulin Unting Dayang Salwani, Awang Adeni GE Environmental Sciences Abundance of lignocellulosic biomass provides a good solution to the demands of energy crops in producing biofuel like biodiesel and bioethanol. In this study, bioethanol was produced from sago hampas via the Simultaneous co-Saccharification and Fermentation (Sc-SF) process, at 2.5% and 5.0% (w/v) solid loadings. The processing step in Sc-SF is virtually similar to that of Simultaneous Saccharification and Fermentation (SSF). However, during Sc-SF, two enzymes, amylase and cellulose, were added for the co-saccharification of sago starch and fiber. In addition, Saccharomyces cerevisiae was used to ferment the sugars in the hydrolysates. The Sc-SF samples were analyzed for carbohydrate residues, ethanol and acetic acid using the dinitrosalicylic (DNS) acid assay and High Performance Liquid Chromatography (HPLC).Results showed that the Sc-SF of the sago hampas showed high efficiencies of hydrolysis and ethanol production within the first six hours of fermentation. Highest glucose production was at 37.86 g/l for the 5.0% sago hampas load and 17.47 g/l for 2.5% sago hampas load. The highest ethanol production was observed in the broth with 5.0% sago hampas, with a theoretical yield of 80.50%. Meanwhile, the highest bioethanol yield in the sample with 2.5% sago hampas was 73.19%. This study indicated that bioethanol production via Sc-SF from starch rich agricultural residues such as sago hampas is feasible. International Commission of Agricultural and Biosystems Engineering 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10732/1/Micky.pdf Micky, Vincent and Empina, Jabang and Norizawati, Muhamad Nur and Ennry, Esut and Leo, Bulin Unting and Dayang Salwani, Awang Adeni (2015) Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production. Agricultural Engineering International: CIGR Journal, 17 (2). pp. 160-167. ISSN 1682-1130 http://www.cigrjournal.org
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
spellingShingle GE Environmental Sciences
Micky, Vincent
Empina, Jabang
Norizawati, Muhamad Nur
Ennry, Esut
Leo, Bulin Unting
Dayang Salwani, Awang Adeni
Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
description Abundance of lignocellulosic biomass provides a good solution to the demands of energy crops in producing biofuel like biodiesel and bioethanol. In this study, bioethanol was produced from sago hampas via the Simultaneous co-Saccharification and Fermentation (Sc-SF) process, at 2.5% and 5.0% (w/v) solid loadings. The processing step in Sc-SF is virtually similar to that of Simultaneous Saccharification and Fermentation (SSF). However, during Sc-SF, two enzymes, amylase and cellulose, were added for the co-saccharification of sago starch and fiber. In addition, Saccharomyces cerevisiae was used to ferment the sugars in the hydrolysates. The Sc-SF samples were analyzed for carbohydrate residues, ethanol and acetic acid using the dinitrosalicylic (DNS) acid assay and High Performance Liquid Chromatography (HPLC).Results showed that the Sc-SF of the sago hampas showed high efficiencies of hydrolysis and ethanol production within the first six hours of fermentation. Highest glucose production was at 37.86 g/l for the 5.0% sago hampas load and 17.47 g/l for 2.5% sago hampas load. The highest ethanol production was observed in the broth with 5.0% sago hampas, with a theoretical yield of 80.50%. Meanwhile, the highest bioethanol yield in the sample with 2.5% sago hampas was 73.19%. This study indicated that bioethanol production via Sc-SF from starch rich agricultural residues such as sago hampas is feasible.
format Article
author Micky, Vincent
Empina, Jabang
Norizawati, Muhamad Nur
Ennry, Esut
Leo, Bulin Unting
Dayang Salwani, Awang Adeni
author_facet Micky, Vincent
Empina, Jabang
Norizawati, Muhamad Nur
Ennry, Esut
Leo, Bulin Unting
Dayang Salwani, Awang Adeni
author_sort Micky, Vincent
title Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
title_short Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
title_full Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
title_fullStr Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
title_full_unstemmed Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
title_sort simultaneous co-saccharification and fermentation of sago hampas for bioethanol production
publisher International Commission of Agricultural and Biosystems Engineering
publishDate 2015
url http://ir.unimas.my/id/eprint/10732/1/Micky.pdf
http://ir.unimas.my/id/eprint/10732/
http://www.cigrjournal.org
_version_ 1724078478588903424
score 13.211869

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