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Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats

Escherichia coli (E. coli) 0157:H7 and Salmonella typhimurium (s. typhimurium) are foodborne pathogens that cause food poisoning outbreaks since decades ago. Studies have shown that exotic meats have the potential to be contaminated by both pathogens. A total of 14 exotic meat samples namely wild bo...

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Bibliographic Details
Main Author: Tan, Mei Chian
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/10619/1/Mei%20Chian%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10619/4/Tan%20Mei%20Chian%20ft.pdf
http://ir.unimas.my/id/eprint/10619/
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spelling my.unimas.ir.106192024-04-01T03:06:10Z http://ir.unimas.my/id/eprint/10619/ Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats Tan, Mei Chian Q Science (General) QR Microbiology Escherichia coli (E. coli) 0157:H7 and Salmonella typhimurium (s. typhimurium) are foodborne pathogens that cause food poisoning outbreaks since decades ago. Studies have shown that exotic meats have the potential to be contaminated by both pathogens. A total of 14 exotic meat samples namely wild boar (n=3), snake (n=3), soft-shelled turtle (n=3), frog (n=3), deer (n=l) and squirrel (n=l) were purchased and collected from wet markets, food court, restaurant and individuals for the present study. The isolation of E. coli and Salmonella spp. from the collected samples were accomplished through selective plating on EMBA and XLD agar respectively while polymerase chain reaction were used to detect the presence of E. coli 0157:H7 and S. typhimurium in the collected samples. The findings showed that 100% (14114) of the samples showed appearance of E. coli colonies on EMBA while 78.57% (11/14) of the samples showed appearance of Salmonella colonies on XLD agar. Both E. coli 0157:H7 and S. typhimurium were not detected from the collected samples through polymerase chain reaction. However, the results cannot prove that exotic meats are safe to be consumed. Hence, more related studies are required to further assess the microbial risk on consuming exotic meats and ensure food safety. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10619/1/Mei%20Chian%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/10619/4/Tan%20Mei%20Chian%20ft.pdf Tan, Mei Chian (2015) Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Tan, Mei Chian
Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
description Escherichia coli (E. coli) 0157:H7 and Salmonella typhimurium (s. typhimurium) are foodborne pathogens that cause food poisoning outbreaks since decades ago. Studies have shown that exotic meats have the potential to be contaminated by both pathogens. A total of 14 exotic meat samples namely wild boar (n=3), snake (n=3), soft-shelled turtle (n=3), frog (n=3), deer (n=l) and squirrel (n=l) were purchased and collected from wet markets, food court, restaurant and individuals for the present study. The isolation of E. coli and Salmonella spp. from the collected samples were accomplished through selective plating on EMBA and XLD agar respectively while polymerase chain reaction were used to detect the presence of E. coli 0157:H7 and S. typhimurium in the collected samples. The findings showed that 100% (14114) of the samples showed appearance of E. coli colonies on EMBA while 78.57% (11/14) of the samples showed appearance of Salmonella colonies on XLD agar. Both E. coli 0157:H7 and S. typhimurium were not detected from the collected samples through polymerase chain reaction. However, the results cannot prove that exotic meats are safe to be consumed. Hence, more related studies are required to further assess the microbial risk on consuming exotic meats and ensure food safety.
format Final Year Project Report
author Tan, Mei Chian
author_facet Tan, Mei Chian
author_sort Tan, Mei Chian
title Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
title_short Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
title_full Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
title_fullStr Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
title_full_unstemmed Investigation of escherichia coli, escherichia coli O157:H7 and salmonella typhimurium in various exotic meats
title_sort investigation of escherichia coli, escherichia coli o157:h7 and salmonella typhimurium in various exotic meats
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10619/1/Mei%20Chian%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10619/4/Tan%20Mei%20Chian%20ft.pdf
http://ir.unimas.my/id/eprint/10619/
_version_ 1795293748353040384
score 13.211869

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