Preliminary study on larvae production of green spotted puffer, tetraodon nigroviridis

Scientific name for green-spotted puffer is Tetraodon nigroviridis and this species belongs to the family of Tetraodontidae. T. nigroviridis is distributed along the brackish water, mangrove area, river and flood plains. T. nigroviridis is importance in genomic study and also famous as ornamental...

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Bibliographic Details
Main Author: Nur Afifah Hanun, binti Ismail
Format: E-LPTA
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
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Online Access:http://ir.unimas.my/id/eprint/10524/1/Preliminary%20Study%20On%20Larvae%20Production%20of%20Green%20Spotted%20Puffer%2C%20Tetraodon%20nigroviridis%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10524/2/Preliminary%20Study%20On%20Larvae%20Production%20of%20Green%20Spotted%20Puffer%2C%20Tetraodon%20nigroviridis%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/10524/
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Summary:Scientific name for green-spotted puffer is Tetraodon nigroviridis and this species belongs to the family of Tetraodontidae. T. nigroviridis is distributed along the brackish water, mangrove area, river and flood plains. T. nigroviridis is importance in genomic study and also famous as ornamental fish. The morphology of T. nigroviridis was examined according to their body weight, standard length and total length. Range of body weight for male T. nigroviridis is 74.92±30.1 g and for female is 106.00±36.96 g. In larvae production, T. nigroviridis was cultured in the laboratory and the artificial breeding was induced by Ovaprim hormone. Ovaprim hormone was used in ornamental finfish brood stock only and it was injected according to the fish body weight. Chromatography analyses (TLC, HPLC and LC-MS) were carried out to analyse the toxic level in the different tissues of brood stock of T. nigroviridis. Among the tissues, egg was found to have the highest concentration of TTX with the result 1330.91 MU/g, followed by intestine and muscle. The baseline data for culturing T. nigroviridis in captivity can be used as a guideline for larvae production in the future. On the other side, the TTX data in T. nigroviridis tissues could be important information and can be used in order to mitigate puffer fish poisoning cases especially in East Malaysia water.