Establishment of axenic culture and callus induction of synsepalum dulcificum daniel (miracle berry)
Synsepalum dulcificum is a slow growing shrub from family Sapotaceae. The fruits contain high medicinal values which aid in chronic diseases due to the presence of active component Miraculin, a taste modifying properties. The current research emphasizes on the axenic cultures establishment and cal...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10514/1/Hong%20Wee%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/10514/4/Tan%20Hong%20Wee%20%20ft.pdf http://ir.unimas.my/id/eprint/10514/ |
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| Summary: | Synsepalum dulcificum is a slow growing shrub from family Sapotaceae. The fruits contain high medicinal
values which aid in chronic diseases due to the presence of active component Miraculin, a taste modifying
properties. The current research emphasizes on the axenic cultures establishment and callus induction of
Synsepalum dulcificum from leaf and seed explants. The sterilized leaf explants were obtained from both 25%
Clorox® solution (Sodium hypochlorite) which produced 91.7% axenic cultures and 25% CaOCI solution
(Calcium hypochlorite) that produced 94.4% axenic cultures with 20 minutes exposure time upon addition of
1% Benomyl solution respectively. The sterilized leaf explants were cultured onto Murashige & Skoog (MS)
media supplemented with various concentrations of 2,4- Dichlorophenoxyacetic Acid (2,4-D), and Picloram.
No callus was induced despite some of the leaf explants remained viable after certain period of time and most
of the leaf explants results in browning conditions whereas callus was induced from seeds supplemented with
2.0 mg/L of 2,4-D after 3 weeks of culturing. The poor callus induction from leaves was highly due to the
presence of high poly-phenolic compounds within the miracle berry plants. Future work should utilize
different PGR‟s at different concentrations and also the use of different explants. |
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