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Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)

Orthosiphon aristatus (Blume) Miq. is a perennial herb under the mint family, Lamiaceae. The study on O. aristatus was conducted with several aims to accomplish which include (1) to study the breeding system of O. aristatus through artificial pollination, (2) to determine the flower developmental at...

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Main Author: Nur Karimah, Mohamad
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/10503/1/NUR%20KARIMAH.pdf
http://ir.unimas.my/id/eprint/10503/4/NUR%20KARIMAH%20full.pdf
http://ir.unimas.my/id/eprint/10503/
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spelling my.unimas.ir.105032024-02-16T09:03:21Z http://ir.unimas.my/id/eprint/10503/ Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.) Nur Karimah, Mohamad SB Plant culture Orthosiphon aristatus (Blume) Miq. is a perennial herb under the mint family, Lamiaceae. The study on O. aristatus was conducted with several aims to accomplish which include (1) to study the breeding system of O. aristatus through artificial pollination, (2) to determine the flower developmental at which the pollen is most viable, (3) to investigate the potential of flavonoids as phytochemical markers, and (4) to determine whether there is any significant effect of different drying conditions on the flavonoid profiles of O. aristatus. In artificial pollination tests, two tests conducted which were self-pollination and cross pollination. All artificial pollination tests were considered as successful if seeds were developed. Pollen viability test were conducted using in vitro germination method and the pollens were collected from three different developmental stages; pre-anthesis, anthesis and post-anthesis. Whilst, for the phytochemical marker analysis of flavonoids, two dimensional paper chromatography (PC) was utilised as the separation technique. Retention factor (Rf) value was calculated for each compound observed and the flavonoid profiles of OAV-1 and OAV-2 were compared. Lastly, the different drying conditions were imposed on the O. aristatus samples to see if there is any significant effect on the flavonoids content of the leaf samples. From the study conducted, O. aristatus was suggested to be a pre-dominantly self-pollinated species with the support of pollen viability test that indicated viable pollen can be observed during pre-anthesis stage. The flavonoid profiles of OAV-1 and OAV-2 showed polymorphism which can be used as marker in breeding program. Drying quality test revealed that temperature plays important factor in the flavonoid contents of O. aristatus C) caused degradation of flavonoid contents Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10503/1/NUR%20KARIMAH.pdf text en http://ir.unimas.my/id/eprint/10503/4/NUR%20KARIMAH%20full.pdf Nur Karimah, Mohamad (2015) Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.). [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic SB Plant culture
spellingShingle SB Plant culture
Nur Karimah, Mohamad
Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
description Orthosiphon aristatus (Blume) Miq. is a perennial herb under the mint family, Lamiaceae. The study on O. aristatus was conducted with several aims to accomplish which include (1) to study the breeding system of O. aristatus through artificial pollination, (2) to determine the flower developmental at which the pollen is most viable, (3) to investigate the potential of flavonoids as phytochemical markers, and (4) to determine whether there is any significant effect of different drying conditions on the flavonoid profiles of O. aristatus. In artificial pollination tests, two tests conducted which were self-pollination and cross pollination. All artificial pollination tests were considered as successful if seeds were developed. Pollen viability test were conducted using in vitro germination method and the pollens were collected from three different developmental stages; pre-anthesis, anthesis and post-anthesis. Whilst, for the phytochemical marker analysis of flavonoids, two dimensional paper chromatography (PC) was utilised as the separation technique. Retention factor (Rf) value was calculated for each compound observed and the flavonoid profiles of OAV-1 and OAV-2 were compared. Lastly, the different drying conditions were imposed on the O. aristatus samples to see if there is any significant effect on the flavonoids content of the leaf samples. From the study conducted, O. aristatus was suggested to be a pre-dominantly self-pollinated species with the support of pollen viability test that indicated viable pollen can be observed during pre-anthesis stage. The flavonoid profiles of OAV-1 and OAV-2 showed polymorphism which can be used as marker in breeding program. Drying quality test revealed that temperature plays important factor in the flavonoid contents of O. aristatus C) caused degradation of flavonoid contents
format Final Year Project Report
author Nur Karimah, Mohamad
author_facet Nur Karimah, Mohamad
author_sort Nur Karimah, Mohamad
title Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
title_short Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
title_full Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
title_fullStr Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
title_full_unstemmed Breeding System and Phytochemical Analysis of Orthosiphon aristatus Blume (Miq.)
title_sort breeding system and phytochemical analysis of orthosiphon aristatus blume (miq.)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10503/1/NUR%20KARIMAH.pdf
http://ir.unimas.my/id/eprint/10503/4/NUR%20KARIMAH%20full.pdf
http://ir.unimas.my/id/eprint/10503/
_version_ 1792160580653547520
score 13.211869

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