Study of toxin production of microcystis under laboratory condition with different nutrient treatment

Aquaculture is an important industries in Malaysia, however, cyanobacterial blooms with potential toxins are problematic in pond water management in aquaculture. In this study, potential toxin-producing cyanobacteria, Microcystis sp. were isolated from selected aquaculture ponds in Indigenous Fisher...

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Main Author: Lee, Li Keat
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
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Online Access:http://ir.unimas.my/id/eprint/10424/1/Study%20of%20Toxin%20Production%20of%20Microcystis%20Under%20Laboratory%20Condition%20With%20Different%20Nutrient%20Treatment%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10424/8/Lee%20Li%20Keat%20ft.pdf
http://ir.unimas.my/id/eprint/10424/
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spelling my.unimas.ir.104242024-02-20T07:07:32Z http://ir.unimas.my/id/eprint/10424/ Study of toxin production of microcystis under laboratory condition with different nutrient treatment Lee, Li Keat SH Aquaculture. Fisheries. Angling Aquaculture is an important industries in Malaysia, however, cyanobacterial blooms with potential toxins are problematic in pond water management in aquaculture. In this study, potential toxin-producing cyanobacteria, Microcystis sp. were isolated from selected aquaculture ponds in Indigenous Fisheries Production and Research Center, Tarat, Sarawak. The isolated Microcystis sp. were successfully maintained and mass culture using BG11 medium under laboratory condition. In-situ and ex-situ water parameter of selected aquaculture pond were recorded and microcystins concentration were detected in bloom-forming ponds using HPLC analysis. There are no significant relation found between chl-a concentration and microcystins concentration of pond samples and culture samples. Meanwhile, in nutrient experiment using different N:P (nitrogen:phosphorus) ratio, NP15(15:1 ratio) give the best growth performance of Microcystis sp. with highest optical density and overall specific growth rate. Highest microcystins concentration was detected in sample with N:P ratio 15:1 at 8.510 μg mg-1. In this experiment, a relation between microcystins production and growth of Microcystis in term of growth density and growth rate was observed but no relation was observed between nitrate concentration and microcystins production. Nevertheless, N:P ratio = 15 was found to be the optimum nutrient ratio for growth of Microcystis sp. and highest microcystins content was detected. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/10424/1/Study%20of%20Toxin%20Production%20of%20Microcystis%20Under%20Laboratory%20Condition%20With%20Different%20Nutrient%20Treatment%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/10424/8/Lee%20Li%20Keat%20ft.pdf Lee, Li Keat (2015) Study of toxin production of microcystis under laboratory condition with different nutrient treatment. [Final Year Project Report]
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Lee, Li Keat
Study of toxin production of microcystis under laboratory condition with different nutrient treatment
description Aquaculture is an important industries in Malaysia, however, cyanobacterial blooms with potential toxins are problematic in pond water management in aquaculture. In this study, potential toxin-producing cyanobacteria, Microcystis sp. were isolated from selected aquaculture ponds in Indigenous Fisheries Production and Research Center, Tarat, Sarawak. The isolated Microcystis sp. were successfully maintained and mass culture using BG11 medium under laboratory condition. In-situ and ex-situ water parameter of selected aquaculture pond were recorded and microcystins concentration were detected in bloom-forming ponds using HPLC analysis. There are no significant relation found between chl-a concentration and microcystins concentration of pond samples and culture samples. Meanwhile, in nutrient experiment using different N:P (nitrogen:phosphorus) ratio, NP15(15:1 ratio) give the best growth performance of Microcystis sp. with highest optical density and overall specific growth rate. Highest microcystins concentration was detected in sample with N:P ratio 15:1 at 8.510 μg mg-1. In this experiment, a relation between microcystins production and growth of Microcystis in term of growth density and growth rate was observed but no relation was observed between nitrate concentration and microcystins production. Nevertheless, N:P ratio = 15 was found to be the optimum nutrient ratio for growth of Microcystis sp. and highest microcystins content was detected.
format Final Year Project Report
author Lee, Li Keat
author_facet Lee, Li Keat
author_sort Lee, Li Keat
title Study of toxin production of microcystis under laboratory condition with different nutrient treatment
title_short Study of toxin production of microcystis under laboratory condition with different nutrient treatment
title_full Study of toxin production of microcystis under laboratory condition with different nutrient treatment
title_fullStr Study of toxin production of microcystis under laboratory condition with different nutrient treatment
title_full_unstemmed Study of toxin production of microcystis under laboratory condition with different nutrient treatment
title_sort study of toxin production of microcystis under laboratory condition with different nutrient treatment
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/10424/1/Study%20of%20Toxin%20Production%20of%20Microcystis%20Under%20Laboratory%20Condition%20With%20Different%20Nutrient%20Treatment%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10424/8/Lee%20Li%20Keat%20ft.pdf
http://ir.unimas.my/id/eprint/10424/
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score 13.211869