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Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak

Sarawak state government’s aspiration to establish one million hectares of planted forests by the year 2020 has only achieved 25 % thus far. It is estimated that at least 30 million of seedlings are needed for annual planting and reforestation. One of the problems causing the slow rate in the esta...

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Main Authors: Pang Shek, Ling, Ho Wei, Seng
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
SB Plant culture
SD Forestry
Online Access:http://ir.unimas.my/id/eprint/10233/1/Inter%20Simple%20Sequence%20Repeat%20%28ISSR%29%20Analysis%20of%20Neolamarckia%20cadamba%20in%20Sarawak%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10233/
http://www.academia.edu/14200065/Inter_simple_sequence_repeat_ISSR_analysis_of_Neolamarckia_cadamba_in_Sarawak
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spelling my.unimas.ir.102332016-01-18T07:32:26Z http://ir.unimas.my/id/eprint/10233/ Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak Pang Shek, Ling Ho Wei, Seng SB Plant culture SD Forestry Sarawak state government’s aspiration to establish one million hectares of planted forests by the year 2020 has only achieved 25 % thus far. It is estimated that at least 30 million of seedlings are needed for annual planting and reforestation. One of the problems causing the slow rate in the establishment of planted forest, despite of the huge number of seedlings needed, is the availability of quality planting materials. To overcome this problem, a tree improvement programme focusing on Neolamarckia cadamba or locally known as Kelampayan was initiated in July 2007. The Kelampayan tree improvement programme is a long-term investment to increase the productivity of forest plantations by providing a source of improved planting stock that will improve the tree growth, better form and wood quality as well as pest and disease resistance. To date, two Kelampayan provenance trials have been established since 2008 in Landeh and Niah, Sarawak. This study is directed at the understanding of the genetic diversity and relatedness of the Kelampayan planted in Block 4 at the Landeh provenance trial using inter simple sequence repeat (ISSR) markers. A total of 34 ISSR markers commonly used in plant genotyping were screened using Kelampayan DNA. Out of these markers, 11 were found to be polymorphic and reproducible. PCR optimizations were carried out on these selected 11 ISSR markers. Genotyping of the Kelampayan using the selected ISSR markers is performed at optimal temperatures (Ta) varying from 44 to 61C. The optimum conditions for these ISSR are as follow: 2ng of DNA template, 2mM MgCl2, 0.5 U Taq DNA polymerase, 0.2mM dNTPs and 10 M primer. The genetic information obtained from this study will be used as one of the parameters for the selection of genetically diverse and nonrelated plus trees from the Landeh provenance trial for future tree improvement programme. 2011 Conference or Workshop Item NonPeerReviewed text en http://ir.unimas.my/id/eprint/10233/1/Inter%20Simple%20Sequence%20Repeat%20%28ISSR%29%20Analysis%20of%20Neolamarckia%20cadamba%20in%20Sarawak%28abstract%29.pdf Pang Shek, Ling and Ho Wei, Seng (2011) Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak. In: 9th Malaysia Genetics Congress (MGC9), 28-30 September 2011, Pullman Hotel, Kuching Sarawak. http://www.academia.edu/14200065/Inter_simple_sequence_repeat_ISSR_analysis_of_Neolamarckia_cadamba_in_Sarawak
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
SD Forestry
spellingShingle SB Plant culture
SD Forestry
Pang Shek, Ling
Ho Wei, Seng
Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
description Sarawak state government’s aspiration to establish one million hectares of planted forests by the year 2020 has only achieved 25 % thus far. It is estimated that at least 30 million of seedlings are needed for annual planting and reforestation. One of the problems causing the slow rate in the establishment of planted forest, despite of the huge number of seedlings needed, is the availability of quality planting materials. To overcome this problem, a tree improvement programme focusing on Neolamarckia cadamba or locally known as Kelampayan was initiated in July 2007. The Kelampayan tree improvement programme is a long-term investment to increase the productivity of forest plantations by providing a source of improved planting stock that will improve the tree growth, better form and wood quality as well as pest and disease resistance. To date, two Kelampayan provenance trials have been established since 2008 in Landeh and Niah, Sarawak. This study is directed at the understanding of the genetic diversity and relatedness of the Kelampayan planted in Block 4 at the Landeh provenance trial using inter simple sequence repeat (ISSR) markers. A total of 34 ISSR markers commonly used in plant genotyping were screened using Kelampayan DNA. Out of these markers, 11 were found to be polymorphic and reproducible. PCR optimizations were carried out on these selected 11 ISSR markers. Genotyping of the Kelampayan using the selected ISSR markers is performed at optimal temperatures (Ta) varying from 44 to 61C. The optimum conditions for these ISSR are as follow: 2ng of DNA template, 2mM MgCl2, 0.5 U Taq DNA polymerase, 0.2mM dNTPs and 10 M primer. The genetic information obtained from this study will be used as one of the parameters for the selection of genetically diverse and nonrelated plus trees from the Landeh provenance trial for future tree improvement programme.
format Conference or Workshop Item
author Pang Shek, Ling
Ho Wei, Seng
author_facet Pang Shek, Ling
Ho Wei, Seng
author_sort Pang Shek, Ling
title Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
title_short Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
title_full Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
title_fullStr Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
title_full_unstemmed Inter Simple Sequence Repeat (ISSR) Analysis of Neolamarckia cadamba in Sarawak
title_sort inter simple sequence repeat (issr) analysis of neolamarckia cadamba in sarawak
publishDate 2011
url http://ir.unimas.my/id/eprint/10233/1/Inter%20Simple%20Sequence%20Repeat%20%28ISSR%29%20Analysis%20of%20Neolamarckia%20cadamba%20in%20Sarawak%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10233/
http://www.academia.edu/14200065/Inter_simple_sequence_repeat_ISSR_analysis_of_Neolamarckia_cadamba_in_Sarawak
_version_ 1644510913366065152
score 13.211869

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