Staff View: DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)

DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)

The molecular genetic marker, minisatellite locus D1S80 (1p35-p36), is a highly polymorphic variable number of tandem repeats (VNTR). Its polymorphic nature allows for phylogenetic studies, forensic analysis, genetic maps construction and paternity testing to be performed. A study of the hypervari...

Full description

Saved in:

Bibliographic Details
Main Authors:	Hairul, Azman Roslan, Nur Hafizah, Azizan
Format:	E-Article
Language:	English
Published:	Researchgate 2009
Subjects:	GE Environmental Sciences
Online Access:	http://ir.unimas.my/id/eprint/10162/1/DNA%20Polymorphism%20of%20D1S80%20Locus%20in%20Modern%20Malay%20Sample%20Population%20of%20Sarawak%20%28DNA%20Polimorfisma%20di%20Lokus%20DIS80%20dalam%20Sampel%20Populasi%20Melayu%20Moden%20Sarawak%20abstract%29.pdf http://ir.unimas.my/id/eprint/10162/ https://www.researchgate.net/publication/237631609_DNA_Polymorphism_of_D1S80_Locus_in_Modern_Malay_Sample_Population_of_Sarawak_DNA_Polimorfisma_di_Lokus_DIS80_dalam_Sampel_Populasi_Melayu_Moden_Sarawak
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.unimas.ir.10162
record_format	eprints
spelling	my.unimas.ir.101622016-01-13T00:43:27Z http://ir.unimas.my/id/eprint/10162/ DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak) Hairul, Azman Roslan Nur Hafizah, Azizan GE Environmental Sciences The molecular genetic marker, minisatellite locus D1S80 (1p35-p36), is a highly polymorphic variable number of tandem repeats (VNTR). Its polymorphic nature allows for phylogenetic studies, forensic analysis, genetic maps construction and paternity testing to be performed. A study of the hypervariable locus D1S80 was conducted to determine the allele frequency and distribution of this locus in modern Malay in Sarawak population. The polymerase chain reaction technique was employed and results were analysed on polyacrylamide gel. A total of seventy-six DNA samples of unrelated Malay individuals in UNIMAS were collected and examined. The VNTR analysis of the D1S80 locus demonstrated the presence of 17 alleles in the Malay population. Allele with the size of 577 bp (27 repeats) was determined to be the most common in the sample population with the frequency of 0.1641, followed by allele with the size of 561 bp (26 repeats) and 529 bp (24 repeats) whose frequency is 0.1172 and 0.1094, respectively. The smallest allele is allele with the size of 465 bp (20 repeats) whereas the largest is allele with the size of 753 bp (38 repeats). The sample population exhibited 57.8% heterozygosity. Researchgate 2009 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/10162/1/DNA%20Polymorphism%20of%20D1S80%20Locus%20in%20Modern%20Malay%20Sample%20Population%20of%20Sarawak%20%28DNA%20Polimorfisma%20di%20Lokus%20DIS80%20dalam%20Sampel%20Populasi%20Melayu%20Moden%20Sarawak%20abstract%29.pdf Hairul, Azman Roslan and Nur Hafizah, Azizan (2009) DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak). Sains Malaysiana, 38 (2). pp. 143-147. ISSN 01266039 https://www.researchgate.net/publication/237631609_DNA_Polymorphism_of_D1S80_Locus_in_Modern_Malay_Sample_Population_of_Sarawak_DNA_Polimorfisma_di_Lokus_DIS80_dalam_Sampel_Populasi_Melayu_Moden_Sarawak
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	GE Environmental Sciences
spellingShingle	GE Environmental Sciences Hairul, Azman Roslan Nur Hafizah, Azizan DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
description	The molecular genetic marker, minisatellite locus D1S80 (1p35-p36), is a highly polymorphic variable number of tandem repeats (VNTR). Its polymorphic nature allows for phylogenetic studies, forensic analysis, genetic maps construction and paternity testing to be performed. A study of the hypervariable locus D1S80 was conducted to determine the allele frequency and distribution of this locus in modern Malay in Sarawak population. The polymerase chain reaction technique was employed and results were analysed on polyacrylamide gel. A total of seventy-six DNA samples of unrelated Malay individuals in UNIMAS were collected and examined. The VNTR analysis of the D1S80 locus demonstrated the presence of 17 alleles in the Malay population. Allele with the size of 577 bp (27 repeats) was determined to be the most common in the sample population with the frequency of 0.1641, followed by allele with the size of 561 bp (26 repeats) and 529 bp (24 repeats) whose frequency is 0.1172 and 0.1094, respectively. The smallest allele is allele with the size of 465 bp (20 repeats) whereas the largest is allele with the size of 753 bp (38 repeats). The sample population exhibited 57.8% heterozygosity.
format	E-Article
author	Hairul, Azman Roslan Nur Hafizah, Azizan
author_facet	Hairul, Azman Roslan Nur Hafizah, Azizan
author_sort	Hairul, Azman Roslan
title	DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
title_short	DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
title_full	DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
title_fullStr	DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
title_full_unstemmed	DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)
title_sort	dna polymorphism of d1s80 locus in modern malay sample population of sarawak (dna polimorfisma di lokus dis80 dalam sampel populasi melayu moden sarawak)
publisher	Researchgate
publishDate	2009
url	http://ir.unimas.my/id/eprint/10162/1/DNA%20Polymorphism%20of%20D1S80%20Locus%20in%20Modern%20Malay%20Sample%20Population%20of%20Sarawak%20%28DNA%20Polimorfisma%20di%20Lokus%20DIS80%20dalam%20Sampel%20Populasi%20Melayu%20Moden%20Sarawak%20abstract%29.pdf http://ir.unimas.my/id/eprint/10162/ https://www.researchgate.net/publication/237631609_DNA_Polymorphism_of_D1S80_Locus_in_Modern_Malay_Sample_Population_of_Sarawak_DNA_Polimorfisma_di_Lokus_DIS80_dalam_Sampel_Populasi_Melayu_Moden_Sarawak
_version_	1644510893769228288
score	13.211869

DNA Polymorphism of D1S80 Locus in Modern Malay Sample Population of Sarawak (DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi Melayu Moden Sarawak)

Similar Items