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Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay

This study has evaluated the use of a commercially available Rainbow agar O157TM and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157TM was found to be selective and sensitiv...

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Main Authors: Son, Radu, Gulam, Rusul, Ooi Wai, Ling, Endang, Purwati, Maimunah, Mustakim, Samuel, Lihan
Format: Article
Language:English
Published: UPM 2000
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/10109/1/Radu.pdf
http://ir.unimas.my/id/eprint/10109/
https://www.researchgate.net/publication/12300897_Rapid_isolation_and_detection_of_Escherichia_coli_O157H7_by_use_of_rainbow_agar_O157_and_PCR_assay
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id my.unimas.ir.10109
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spelling my.unimas.ir.101092021-07-14T17:02:28Z http://ir.unimas.my/id/eprint/10109/ Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay Son, Radu Gulam, Rusul Ooi Wai, Ling Endang, Purwati Maimunah, Mustakim Samuel, Lihan QR Microbiology This study has evaluated the use of a commercially available Rainbow agar O157TM and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157TM was found to be selective and sensitive for the screening of the E. coli O157 from artificially and naturally contaminated meat samples. Shiga-like toxin producing E. coli were identified with two primer pairs that amplified fragments of the SLT-I (384 bp) and SLT-II (584 bp). E. coli O157:H7 was serotyped with a primer pair specified for the H7 flagellar gene, which amplify specific DNA fragments (625 bp) from all E. coli O157:H7 strains. The use of Rainbow agar O157TM described allows for the presumptive isolation of E. coli O157 in 24 hours. Identification and confirmation of the presumptive isolates as E. coli O157:H7 by PCR assays require additional 6-8 hours. The above-mentioned screening and identification procedures should prove to be a very useful method since it allows for the specific detection of E. coli O157:H7. UPM 2000 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10109/1/Radu.pdf Son, Radu and Gulam, Rusul and Ooi Wai, Ling and Endang, Purwati and Maimunah, Mustakim and Samuel, Lihan (2000) Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay. The Southeast Asian journal of tropical medicine and public health, 31 (1). pp. 77-79. ISSN 0125-1562 https://www.researchgate.net/publication/12300897_Rapid_isolation_and_detection_of_Escherichia_coli_O157H7_by_use_of_rainbow_agar_O157_and_PCR_assay
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Son, Radu
Gulam, Rusul
Ooi Wai, Ling
Endang, Purwati
Maimunah, Mustakim
Samuel, Lihan
Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
description This study has evaluated the use of a commercially available Rainbow agar O157TM and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157TM was found to be selective and sensitive for the screening of the E. coli O157 from artificially and naturally contaminated meat samples. Shiga-like toxin producing E. coli were identified with two primer pairs that amplified fragments of the SLT-I (384 bp) and SLT-II (584 bp). E. coli O157:H7 was serotyped with a primer pair specified for the H7 flagellar gene, which amplify specific DNA fragments (625 bp) from all E. coli O157:H7 strains. The use of Rainbow agar O157TM described allows for the presumptive isolation of E. coli O157 in 24 hours. Identification and confirmation of the presumptive isolates as E. coli O157:H7 by PCR assays require additional 6-8 hours. The above-mentioned screening and identification procedures should prove to be a very useful method since it allows for the specific detection of E. coli O157:H7.
format Article
author Son, Radu
Gulam, Rusul
Ooi Wai, Ling
Endang, Purwati
Maimunah, Mustakim
Samuel, Lihan
author_facet Son, Radu
Gulam, Rusul
Ooi Wai, Ling
Endang, Purwati
Maimunah, Mustakim
Samuel, Lihan
author_sort Son, Radu
title Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
title_short Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
title_full Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
title_fullStr Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
title_full_unstemmed Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
title_sort rapid isolation and detection of escherichia coli o157h7 by use of rainbow agar o157 and pcr assay
publisher UPM
publishDate 2000
url http://ir.unimas.my/id/eprint/10109/1/Radu.pdf
http://ir.unimas.my/id/eprint/10109/
https://www.researchgate.net/publication/12300897_Rapid_isolation_and_detection_of_Escherichia_coli_O157H7_by_use_of_rainbow_agar_O157_and_PCR_assay
_version_ 1706961315768041472
score 13.211869

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